Detection of gene cis-regulatory element perturbations in single-cell transcriptomes

Detection of gene cis-regulatory element perturbations in single-cell transcriptomes

We introduce poly-adenine CRISPR gRNA-based single-cell RNA-sequencing (pAC-Seq), a method that enables the direct observation of guide RNAs (gRNAs) in scRNA-seq. We use pAC-Seq to assess the phenotypic consequences of CRISPR/Cas9 based alterations of gene cis-regulatory regions. We show that pAC-Se...

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Bibliographic Details
Main Authors: Yeo, Grace Hui Ting, Juez, Oscar, Chen, Qing, Banerjee, Budhaditya, Chu, Lendy, Shen, Max Walt, Sabry, May, Logister, Ive, Sherwood, Richard I., Gifford, David K
Other Authors: Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2021
Online Access:https://hdl.handle.net/1721.1/130516

Internet

https://hdl.handle.net/1721.1/130516

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