CH-[pi] interactions play a central role in protein recognition of carbohydrates/
Thesis: Ph. D., Massachusetts Institute of Technology, Department of Chemistry, February, 2021
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2021
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| Online Access: | https://hdl.handle.net/1721.1/130817 |
| _version_ | 1826198655530434560 |
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| author | Diehl, Roger Christopher. |
| author2 | Laura L. Kiessling. |
| author_facet | Laura L. Kiessling. Diehl, Roger Christopher. |
| author_sort | Diehl, Roger Christopher. |
| collection | MIT |
| description | Thesis: Ph. D., Massachusetts Institute of Technology, Department of Chemistry, February, 2021 |
| first_indexed | 2024-09-23T11:07:51Z |
| format | Thesis |
| id | mit-1721.1/130817 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T11:07:51Z |
| publishDate | 2021 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/1308172021-05-26T03:25:44Z CH-[pi] interactions play a central role in protein recognition of carbohydrates/ Diehl, Roger Christopher. Laura L. Kiessling. Massachusetts Institute of Technology. Department of Chemistry. Massachusetts Institute of Technology. Department of Chemistry Chemistry. Thesis: Ph. D., Massachusetts Institute of Technology, Department of Chemistry, February, 2021 Cataloged from the official PDF of thesis. In title on title page, [pi] appears as lower case Greek letter. Includes bibliographical references. Carbohydrate-protein interactions play a central role in biology, but knowledge of the forces underlying them is limited. Carbohydrates are generally hydrophilic and therefore present unique challenges in their recognition. One underappreciated force involved in carbohydrate-protein interactions is the CH-[pi] interaction, an attractive interaction between the aliphatic protons of a carbohydrate and the [pi] system of an aromatic ring. In this thesis, I examine the fundamental nature, strength, and biological significance of this interaction, largely in the context of a family of carbohydrate-binding proteins known as galectins. In Chapter 1, I review previous knowledge of the forces underlying carbohydrate-binding proteins and the forces they utilize to bind their ligands. In particular, I focus on CH-[pi] interactions and galectins. In Chapter 2, I examine the forces that contribute to CH-[pi] interactions in the context of carbohydrates and aromatic compounds in aqueous solution. I find the CH-[pi] interaction to be electronic in nature, and demonstrate its selectivity between different carbohydrates. In Chapter 3, I determine the contribution of the CH-[pi] interaction to the ligand binding of galectin-3, a human carbohydrate-binding protein of medical significance. The data demonstrate that the CH-[pi] interaction accounts for a majority of the binding energy. In Chapter 4, I explore the biological implications of the CH-[pi] interaction in galectin-3. I demonstrate that the CH-[pi] interaction is critical for the biological activities of galectin-3. In Chapter 5, I propose several directions future researchers could take to extend this work. For three of four directions, I present the progress I have made during my studies. The work contained within this thesis demonstrates that CH-[pi] interactions play a central role in protein-carbohydrate interactions at both a molecular level and a biological level. Understanding the CH-[pi] interaction is key to explaining and predicting the activity of carbohydrate-binding proteins. by Roger Christopher Diehl. Ph. D. Ph.D. Massachusetts Institute of Technology, Department of Chemistry 2021-05-25T18:21:16Z 2021-05-25T18:21:16Z 2021 2021 Thesis https://hdl.handle.net/1721.1/130817 1252627437 eng MIT theses may be protected by copyright. Please reuse MIT thesis content according to the MIT Libraries Permissions Policy, which is available through the URL provided. http://dspace.mit.edu/handle/1721.1/7582 102 pages application/pdf Massachusetts Institute of Technology |
| spellingShingle | Chemistry. Diehl, Roger Christopher. CH-[pi] interactions play a central role in protein recognition of carbohydrates/ |
| title | CH-[pi] interactions play a central role in protein recognition of carbohydrates/ |
| title_full | CH-[pi] interactions play a central role in protein recognition of carbohydrates/ |
| title_fullStr | CH-[pi] interactions play a central role in protein recognition of carbohydrates/ |
| title_full_unstemmed | CH-[pi] interactions play a central role in protein recognition of carbohydrates/ |
| title_short | CH-[pi] interactions play a central role in protein recognition of carbohydrates/ |
| title_sort | ch pi interactions play a central role in protein recognition of carbohydrates |
| topic | Chemistry. |
| url | https://hdl.handle.net/1721.1/130817 |
| work_keys_str_mv | AT diehlrogerchristopher chpiinteractionsplayacentralroleinproteinrecognitionofcarbohydrates |