Monitoring and modeling of lymphocytic leukemia cell bioenergetics reveals decreased ATP synthesis during cell division
© 2020, The Author(s). The energetic demands of a cell are believed to increase during mitosis, but the rates of ATP synthesis and consumption during mitosis have not been quantified. Here, we monitor mitochondrial membrane potential of single lymphocytic leukemia cells and demonstrate that mitochon...
| Main Authors: | , , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Springer Science and Business Media LLC
2021
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| Online Access: | https://hdl.handle.net/1721.1/133549 |
| _version_ | 1811087679057559552 |
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| author | Kang, Joon Ho Katsikis, Georgios Li, Zhaoqi Sapp, Kiera M Stockslager, Max A Lim, Daniel Vander Heiden, Matthew G Yaffe, Michael B Manalis, Scott R Miettinen, Teemu P |
| author2 | Koch Institute for Integrative Cancer Research at MIT |
| author_facet | Koch Institute for Integrative Cancer Research at MIT Kang, Joon Ho Katsikis, Georgios Li, Zhaoqi Sapp, Kiera M Stockslager, Max A Lim, Daniel Vander Heiden, Matthew G Yaffe, Michael B Manalis, Scott R Miettinen, Teemu P |
| author_sort | Kang, Joon Ho |
| collection | MIT |
| description | © 2020, The Author(s). The energetic demands of a cell are believed to increase during mitosis, but the rates of ATP synthesis and consumption during mitosis have not been quantified. Here, we monitor mitochondrial membrane potential of single lymphocytic leukemia cells and demonstrate that mitochondria hyperpolarize from the G2/M transition until the metaphase-anaphase transition. This hyperpolarization was dependent on cyclin-dependent kinase 1 (CDK1) activity. By using an electrical circuit model of mitochondria, we quantify mitochondrial ATP synthesis rates in mitosis from the single-cell time-dynamics of mitochondrial membrane potential. We find that mitochondrial ATP synthesis decreases by approximately 50% during early mitosis and increases back to G2 levels during cytokinesis. Consistently, ATP levels and ATP synthesis are lower in mitosis than in G2 in synchronized cell populations. Overall, our results provide insights into mitotic bioenergetics and suggest that cell division is not a highly energy demanding process. |
| first_indexed | 2024-09-23T13:50:17Z |
| format | Article |
| id | mit-1721.1/133549 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T13:50:17Z |
| publishDate | 2021 |
| publisher | Springer Science and Business Media LLC |
| record_format | dspace |
| spelling | mit-1721.1/1335492023-12-19T20:52:25Z Monitoring and modeling of lymphocytic leukemia cell bioenergetics reveals decreased ATP synthesis during cell division Kang, Joon Ho Katsikis, Georgios Li, Zhaoqi Sapp, Kiera M Stockslager, Max A Lim, Daniel Vander Heiden, Matthew G Yaffe, Michael B Manalis, Scott R Miettinen, Teemu P Koch Institute for Integrative Cancer Research at MIT Massachusetts Institute of Technology. Department of Physics Center for Precision Cancer Medicine Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Mechanical Engineering © 2020, The Author(s). The energetic demands of a cell are believed to increase during mitosis, but the rates of ATP synthesis and consumption during mitosis have not been quantified. Here, we monitor mitochondrial membrane potential of single lymphocytic leukemia cells and demonstrate that mitochondria hyperpolarize from the G2/M transition until the metaphase-anaphase transition. This hyperpolarization was dependent on cyclin-dependent kinase 1 (CDK1) activity. By using an electrical circuit model of mitochondria, we quantify mitochondrial ATP synthesis rates in mitosis from the single-cell time-dynamics of mitochondrial membrane potential. We find that mitochondrial ATP synthesis decreases by approximately 50% during early mitosis and increases back to G2 levels during cytokinesis. Consistently, ATP levels and ATP synthesis are lower in mitosis than in G2 in synchronized cell populations. Overall, our results provide insights into mitotic bioenergetics and suggest that cell division is not a highly energy demanding process. 2021-10-27T19:53:29Z 2021-10-27T19:53:29Z 2020 2021-08-03T16:46:58Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/133549 en 10.1038/S41467-020-18769-Y Nature Communications Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf Springer Science and Business Media LLC Nature |
| spellingShingle | Kang, Joon Ho Katsikis, Georgios Li, Zhaoqi Sapp, Kiera M Stockslager, Max A Lim, Daniel Vander Heiden, Matthew G Yaffe, Michael B Manalis, Scott R Miettinen, Teemu P Monitoring and modeling of lymphocytic leukemia cell bioenergetics reveals decreased ATP synthesis during cell division |
| title | Monitoring and modeling of lymphocytic leukemia cell bioenergetics reveals decreased ATP synthesis during cell division |
| title_full | Monitoring and modeling of lymphocytic leukemia cell bioenergetics reveals decreased ATP synthesis during cell division |
| title_fullStr | Monitoring and modeling of lymphocytic leukemia cell bioenergetics reveals decreased ATP synthesis during cell division |
| title_full_unstemmed | Monitoring and modeling of lymphocytic leukemia cell bioenergetics reveals decreased ATP synthesis during cell division |
| title_short | Monitoring and modeling of lymphocytic leukemia cell bioenergetics reveals decreased ATP synthesis during cell division |
| title_sort | monitoring and modeling of lymphocytic leukemia cell bioenergetics reveals decreased atp synthesis during cell division |
| url | https://hdl.handle.net/1721.1/133549 |
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