Single-cell measurement of plasmid copy number and promoter activity
Accurate measurements of promoter activities are crucial for predictably building genetic systems. Here we report a method to simultaneously count plasmid DNA, RNA transcripts, and protein expression in single living bacteria. From these data, the activity of a promoter in units of RNAP/s can be inf...
| Päätekijät: | , , , , , |
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| Aineistotyyppi: | Artikkeli |
| Kieli: | English |
| Julkaistu: |
Springer Science and Business Media LLC
2021
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| Linkit: | https://hdl.handle.net/1721.1/133600 |