Single-cell measurement of plasmid copy number and promoter activity

Single-cell measurement of plasmid copy number and promoter activity

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Accurate measurements of promoter activities are crucial for predictably building genetic systems. Here we report a method to simultaneously count plasmid DNA, RNA transcripts, and protein expression in single living bacteria. From these data, the activity of a promoter in units of RNAP/s can be inf...

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Bibliographic Details
Main Authors:	Shao, Bin, Rammohan, Jayan, Anderson, Daniel A, Alperovich, Nina, Ross, David, Voigt, Christopher A
Format:	Article
Language:	English
Published:	Springer Science and Business Media LLC 2021
Online Access:	https://hdl.handle.net/1721.1/133600

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