Single-cell measurement of plasmid copy number and promoter activity
Accurate measurements of promoter activities are crucial for predictably building genetic systems. Here we report a method to simultaneously count plasmid DNA, RNA transcripts, and protein expression in single living bacteria. From these data, the activity of a promoter in units of RNAP/s can be inf...
Main Authors: | Shao, Bin, Rammohan, Jayan, Anderson, Daniel A, Alperovich, Nina, Ross, David, Voigt, Christopher A |
---|---|
Format: | Article |
Language: | English |
Published: |
Springer Science and Business Media LLC
2021
|
Online Access: | https://hdl.handle.net/1721.1/133600 |
Similar Items
-
Single-cell measurement of plasmid copy number and promoter activity
by: Shao, Bin, et al.
Published: (2022) -
A plasmid system with tunable copy number
by: Miles V. Rouches, et al.
Published: (2022-07-01) -
Single-cell measurement quality in bits.
by: Jayan Rammohan, et al.
Published: (2022-01-01) -
The cost of copy number in a selfish genetic element: the 2-μm plasmid of Saccharomyces cerevisiae.
by: Harrison, E, et al.
Published: (2012) -
Inducible plasmid copy number control for synthetic biology in commonly used E. coli strains
by: Shivang Hina-Nilesh Joshi, et al.
Published: (2022-11-01)