Precise Manipulation of Chromosomes in Vivo Enables Genome-Wide Codon Replacement

Precise Manipulation of Chromosomes in Vivo Enables Genome-Wide Codon Replacement

We present genome engineering technologies that are capable of fundamentally reengineering genomes from the nucleotide to the megabase scale. We used multiplex automated genome engineering (MAGE) to site-specifically replace all 314 TAG stop codons with synonymous TAA codons in parallel across 32 Es...

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Bibliographic Details
Main Authors: Isaacs, Farren J, Carr, Peter A, Wang, Harris H, Lajoie, Marc J, Sterling, Bram, Kraal, Laurens, Tolonen, Andrew C, Gianoulis, Tara A, Goodman, Daniel B, Reppas, Nikos B, Emig, Christopher J, Bang, Duhee, Hwang, Samuel J, Jewett, Michael C, Jacobson, Joseph M, Church, George M
Other Authors: Massachusetts Institute of Technology. Center for Bits and Atoms
Format: Article
Language:English
Published: American Association for the Advancement of Science (AAAS) 2021
Online Access:https://hdl.handle.net/1721.1/134637

Internet

https://hdl.handle.net/1721.1/134637

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