A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters

A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters

© 2018 The Author(s). We developed a new way to engineer complex proteins toward multidimensional specifications using a simple, yet scalable, directed evolution strategy. By robotically picking mammalian cells that were identified, under a microscope, as expressing proteins that simultaneously exhi...

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Bibliographic Details
Main Authors: Piatkevich, Kiryl D, Jung, Erica E, Straub, Christoph, Linghu, Changyang, Park, Demian, Suk, Ho-Jun, Hochbaum, Daniel R, Goodwin, Daniel, Pnevmatikakis, Eftychios, Pak, Nikita, Kawashima, Takashi, Yang, Chao-Tsung, Rhoades, Jeffrey L, Shemesh, Or, Asano, Shoh, Yoon, Young-Gyu, Freifeld, Limor, Saulnier, Jessica L, Riegler, Clemens, Engert, Florian, Hughes, Thom, Drobizhev, Mikhail, Szabo, Balint, Ahrens, Misha B, Flavell, Steven W, Sabatini, Bernardo L, Boyden, Edward S
Other Authors: Massachusetts Institute of Technology. Media Laboratory
Format: Article
Language:English
Published: Springer Science and Business Media LLC 2021
Online Access:https://hdl.handle.net/1721.1/134689

Internet

https://hdl.handle.net/1721.1/134689

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