Host-Informed Expression of CRISPR Guide RNA for Genomic Engineering in Komagataella phaffii

Copyright © 2019 American Chemical Society. There is growing interest in the use of nonmodel microorganisms as hosts for biopharmaceutical manufacturing. These hosts require genomic engineering to meet clinically relevant product qualities and titers, but the adaptation of tools for editing genomes,...

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Bibliographic Details
Main Authors: Dalvie, Neil C, Leal, Justin, Whittaker, Charles A, Yang, Yuchen, Brady, Joseph R, Love, Kerry R, Love, J Christopher
Format: Article
Language:English
Published: American Chemical Society (ACS) 2021
Online Access:https://hdl.handle.net/1721.1/136627