Highly Parallel Profiling of Cas9 Variant Specificity
© 2020 Elsevier Inc. Schmid-Burgk et al. develop tagmentation-based tag integration site sequencing (TTISS), a rapid, streamlined protocol for analyzing double-strand breaks such as those created by CRISPR nucleases. Using TTISS, they comprehensively assess Cas9 variants, revealing a trade-off betwe...
Main Authors: | , , , , , , |
---|---|
Other Authors: | |
Format: | Article |
Language: | English |
Published: |
Elsevier BV
2021
|
Online Access: | https://hdl.handle.net/1721.1/138391 |