Highly Parallel Profiling of Cas9 Variant Specificity
© 2020 Elsevier Inc. Schmid-Burgk et al. develop tagmentation-based tag integration site sequencing (TTISS), a rapid, streamlined protocol for analyzing double-strand breaks such as those created by CRISPR nucleases. Using TTISS, they comprehensively assess Cas9 variants, revealing a trade-off betwe...
| Main Authors: | , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Elsevier BV
2021
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| Online Access: | https://hdl.handle.net/1721.1/138391 |
| _version_ | 1826213827671228416 |
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| author | Schmid-Burgk, JL Gao, L Li, D Gardner, Z Strecker, J Lash, B Zhang, F |
| author2 | McGovern Institute for Brain Research at MIT |
| author_facet | McGovern Institute for Brain Research at MIT Schmid-Burgk, JL Gao, L Li, D Gardner, Z Strecker, J Lash, B Zhang, F |
| author_sort | Schmid-Burgk, JL |
| collection | MIT |
| description | © 2020 Elsevier Inc. Schmid-Burgk et al. develop tagmentation-based tag integration site sequencing (TTISS), a rapid, streamlined protocol for analyzing double-strand breaks such as those created by CRISPR nucleases. Using TTISS, they comprehensively assess Cas9 variants, revealing a trade-off between specificity and activity and identifying LZ3 Cas9, a variant with a unique +1 insertion profile. |
| first_indexed | 2024-09-23T15:55:27Z |
| format | Article |
| id | mit-1721.1/138391 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T15:55:27Z |
| publishDate | 2021 |
| publisher | Elsevier BV |
| record_format | dspace |
| spelling | mit-1721.1/1383912023-06-28T18:13:39Z Highly Parallel Profiling of Cas9 Variant Specificity Schmid-Burgk, JL Gao, L Li, D Gardner, Z Strecker, J Lash, B Zhang, F McGovern Institute for Brain Research at MIT Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Massachusetts Institute of Technology. Department of Biological Engineering Howard Hughes Medical Institute © 2020 Elsevier Inc. Schmid-Burgk et al. develop tagmentation-based tag integration site sequencing (TTISS), a rapid, streamlined protocol for analyzing double-strand breaks such as those created by CRISPR nucleases. Using TTISS, they comprehensively assess Cas9 variants, revealing a trade-off between specificity and activity and identifying LZ3 Cas9, a variant with a unique +1 insertion profile. 2021-12-08T18:21:07Z 2021-12-08T18:21:07Z 2020-05-21 2021-12-08T18:19:15Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/138391 Schmid-Burgk, JL, Gao, L, Li, D, Gardner, Z, Strecker, J et al. 2020. "Highly Parallel Profiling of Cas9 Variant Specificity." Molecular Cell, 78 (4). en 10.1016/j.molcel.2020.02.023 Molecular Cell Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier BV PMC |
| spellingShingle | Schmid-Burgk, JL Gao, L Li, D Gardner, Z Strecker, J Lash, B Zhang, F Highly Parallel Profiling of Cas9 Variant Specificity |
| title | Highly Parallel Profiling of Cas9 Variant Specificity |
| title_full | Highly Parallel Profiling of Cas9 Variant Specificity |
| title_fullStr | Highly Parallel Profiling of Cas9 Variant Specificity |
| title_full_unstemmed | Highly Parallel Profiling of Cas9 Variant Specificity |
| title_short | Highly Parallel Profiling of Cas9 Variant Specificity |
| title_sort | highly parallel profiling of cas9 variant specificity |
| url | https://hdl.handle.net/1721.1/138391 |
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