dCas9 regulator to neutralize competition in CRISPRi circuits

dCas9 regulator to neutralize competition in CRISPRi circuits

CRISPRi-mediated gene regulation allows simultaneous control of many genes. However, highly specific sgRNA-promoter binding is, alone, insufficient to achieve independent transcriptional regulation of multiple targets. Indeed, due to competition for dCas9, the repression ability of one sgRNA changes...

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Bibliographic Details
Main Authors: Huang, Hsin-Ho, Bellato, Massimo, Qian, Yili, Cárdenas, Pablo, Pasotti, Lorenzo, Magni, Paolo, Del Vecchio, Domitilla
Other Authors: Massachusetts Institute of Technology. Department of Mechanical Engineering
Format: Article
Language:English
Published: Springer Science and Business Media LLC 2021
Online Access:https://hdl.handle.net/1721.1/138546

Internet

https://hdl.handle.net/1721.1/138546

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