CRISPR/Cas9 editing to generate a heterozygous COL2A1 p.G1170S human chondrodysplasia iPSC line, MCRIi019-A-2, in a control iPSC line, MCRIi019-A

© 2020 To develop an in vitro disease model of a human chondrodysplasia, we used CRISPR/Cas9 gene editing to generate a heterozygous COL2A1 exon 50 c.3508 GGT > TCA (p.G1170S) mutation in a control human iPSC line. Both the control and COL2A1 mutant lines displayed typical iPSC characteristics, i...

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Main Authors: Kung, Louise HW, Sampurno, Lisa, Yammine, Kathryn M, Graham, Alison, McDonald, Penny, Bateman, John F, Shoulders, Matthew D, Lamandé, Shireen R
格式: 文件
语言:English
出版: Elsevier BV 2022
在线阅读:https://hdl.handle.net/1721.1/141307