Methods for CRISPR Cas12a Multiplexing in Mammalian Systems
Clustered regularly interspaced short palindromic repeat (CRISPR) proteins have been found in many bacterial species, serving as a defense mechanism to defend the cell from bacteriophage invasion. These proteins are guided to cleave a specific nucleic acid sequence through the binding of a crRNA hai...
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Thesis |
| Published: |
Massachusetts Institute of Technology
2022
|
| Online Access: | https://hdl.handle.net/1721.1/142812 |