High-throughput functional variant screens via in vivo production of single-stranded DNA

High-throughput functional variant screens via in vivo production of single-stranded DNA

<jats:title>Significance</jats:title> <jats:p>We report a methodology for the pooled construction of mutants bearing precise genomic sequence variations and multiplex phenotypic characterization of these mutants using next-generation sequencing (NGS). Unlike existing tec...

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Bibliographic Details
Main Authors: Schubert, Max G, Goodman, Daniel B, Wannier, Timothy M, Kaur, Divjot, Farzadfard, Fahim, Lu, Timothy K, Shipman, Seth L, Church, George M
Other Authors: Massachusetts Institute of Technology. Research Laboratory of Electronics
Format: Article
Language:English
Published: Proceedings of the National Academy of Sciences 2022
Online Access:https://hdl.handle.net/1721.1/143760

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https://hdl.handle.net/1721.1/143760

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