High-throughput functional variant screens via in vivo production of single-stranded DNA
<jats:title>Significance</jats:title>
<jats:p>We report a methodology for the pooled construction of mutants bearing precise genomic sequence variations and multiplex phenotypic characterization of these mutants using next-generation sequencing (NGS). Unlike existing tec...
Full description
Bibliographic Details
| Main Authors: |
Schubert, Max G,
Goodman, Daniel B,
Wannier, Timothy M,
Kaur, Divjot,
Farzadfard, Fahim,
Lu, Timothy K,
Shipman, Seth L,
Church, George M |
|---|
| Other Authors: |
Massachusetts Institute of Technology. Research Laboratory of Electronics |
|---|
| Format: | Article
|
|---|
| Language: | English |
|---|
| Published: |
Proceedings of the National Academy of Sciences
2022
|
|---|
| Online Access: | https://hdl.handle.net/1721.1/143760
|
|---|