Permitted and restricted steps of human kinetochore assembly in mitotic cell extracts
<jats:p> This work represents the first systematic analysis of kinetochore assembly in human mitotic cell extracts. Using recombinant and native proteins, we show that only a few assembly steps are permitted, suggesting that multiple mechanisms guard against the spurious formation of microtubu...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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American Society for Cell Biology (ASCB)
2022
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| Online Access: | https://hdl.handle.net/1721.1/146774 |
| _version_ | 1826193694031609856 |
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| author | Tarasovetc, Ekaterina V Allu, Praveen Kumar Wimbish, Robert T DeLuca, Jennifer G Cheeseman, Iain M Black, Ben E Grishchuk, Ekaterina L |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Tarasovetc, Ekaterina V Allu, Praveen Kumar Wimbish, Robert T DeLuca, Jennifer G Cheeseman, Iain M Black, Ben E Grishchuk, Ekaterina L |
| author_sort | Tarasovetc, Ekaterina V |
| collection | MIT |
| description | <jats:p> This work represents the first systematic analysis of kinetochore assembly in human mitotic cell extracts. Using recombinant and native proteins, we show that only a few assembly steps are permitted, suggesting that multiple mechanisms guard against the spurious formation of microtubule-binding kinetochore particles in the cytoplasm of mitotic cells. </jats:p> |
| first_indexed | 2024-09-23T09:43:11Z |
| format | Article |
| id | mit-1721.1/146774 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T09:43:11Z |
| publishDate | 2022 |
| publisher | American Society for Cell Biology (ASCB) |
| record_format | dspace |
| spelling | mit-1721.1/1467742022-12-08T03:38:57Z Permitted and restricted steps of human kinetochore assembly in mitotic cell extracts Tarasovetc, Ekaterina V Allu, Praveen Kumar Wimbish, Robert T DeLuca, Jennifer G Cheeseman, Iain M Black, Ben E Grishchuk, Ekaterina L Massachusetts Institute of Technology. Department of Biology <jats:p> This work represents the first systematic analysis of kinetochore assembly in human mitotic cell extracts. Using recombinant and native proteins, we show that only a few assembly steps are permitted, suggesting that multiple mechanisms guard against the spurious formation of microtubule-binding kinetochore particles in the cytoplasm of mitotic cells. </jats:p> 2022-12-07T15:20:40Z 2022-12-07T15:20:40Z 2021 2022-12-07T15:15:42Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/146774 Tarasovetc, Ekaterina V, Allu, Praveen Kumar, Wimbish, Robert T, DeLuca, Jennifer G, Cheeseman, Iain M et al. 2021. "Permitted and restricted steps of human kinetochore assembly in mitotic cell extracts." Molecular Biology of the Cell, 32 (13). en 10.1091/MBC.E20-07-0461 Molecular Biology of the Cell Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf American Society for Cell Biology (ASCB) American Society for Cell Biology |
| spellingShingle | Tarasovetc, Ekaterina V Allu, Praveen Kumar Wimbish, Robert T DeLuca, Jennifer G Cheeseman, Iain M Black, Ben E Grishchuk, Ekaterina L Permitted and restricted steps of human kinetochore assembly in mitotic cell extracts |
| title | Permitted and restricted steps of human kinetochore assembly in mitotic cell extracts |
| title_full | Permitted and restricted steps of human kinetochore assembly in mitotic cell extracts |
| title_fullStr | Permitted and restricted steps of human kinetochore assembly in mitotic cell extracts |
| title_full_unstemmed | Permitted and restricted steps of human kinetochore assembly in mitotic cell extracts |
| title_short | Permitted and restricted steps of human kinetochore assembly in mitotic cell extracts |
| title_sort | permitted and restricted steps of human kinetochore assembly in mitotic cell extracts |
| url | https://hdl.handle.net/1721.1/146774 |
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