The sound of silence: Transgene silencing in mammalian cell engineering
To elucidate principles operating in native biological systems and to develop novel biotechnologies, synthetic biology aims to build and integrate synthetic gene circuits within native transcriptional networks. The utility of synthetic gene circuits for cell engineering relies on the ability to cont...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Elsevier BV
2023
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| Online Access: | https://hdl.handle.net/1721.1/147792 |
| _version_ | 1811093229016186880 |
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| author | Cabrera, Alan Edelstein, Hailey I Glykofrydis, Fokion Love, Kasey S Palacios, Sebastian Tycko, Josh Zhang, Meng Lensch, Sarah Shields, Cara E Livingston, Mark Weiss, Ron Zhao, Huimin Haynes, Karmella A Morsut, Leonardo Chen, Yvonne Y Khalil, Ahmad S Wong, Wilson W Collins, James J Rosser, Susan J Polizzi, Karen Elowitz, Michael B Fussenegger, Martin Hilton, Isaac B Leonard, Joshua N Bintu, Lacramioara Galloway, Kate E Deans, Tara L |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Cabrera, Alan Edelstein, Hailey I Glykofrydis, Fokion Love, Kasey S Palacios, Sebastian Tycko, Josh Zhang, Meng Lensch, Sarah Shields, Cara E Livingston, Mark Weiss, Ron Zhao, Huimin Haynes, Karmella A Morsut, Leonardo Chen, Yvonne Y Khalil, Ahmad S Wong, Wilson W Collins, James J Rosser, Susan J Polizzi, Karen Elowitz, Michael B Fussenegger, Martin Hilton, Isaac B Leonard, Joshua N Bintu, Lacramioara Galloway, Kate E Deans, Tara L |
| author_sort | Cabrera, Alan |
| collection | MIT |
| description | To elucidate principles operating in native biological systems and to develop novel biotechnologies, synthetic biology aims to build and integrate synthetic gene circuits within native transcriptional networks. The utility of synthetic gene circuits for cell engineering relies on the ability to control the expression of all constituent transgene components. Transgene silencing, defined as the loss of expression over time, persists as an obstacle for engineering primary cells and stem cells with transgenic cargos. In this review, we highlight the challenge that transgene silencing poses to the robust engineering of mammalian cells, outline potential molecular mechanisms of silencing, and present approaches for preventing transgene silencing. We conclude with a perspective identifying future research directions for improving the performance of synthetic gene circuits. |
| first_indexed | 2024-09-23T15:41:57Z |
| format | Article |
| id | mit-1721.1/147792 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T15:41:57Z |
| publishDate | 2023 |
| publisher | Elsevier BV |
| record_format | dspace |
| spelling | mit-1721.1/1477922023-02-01T03:07:15Z The sound of silence: Transgene silencing in mammalian cell engineering Cabrera, Alan Edelstein, Hailey I Glykofrydis, Fokion Love, Kasey S Palacios, Sebastian Tycko, Josh Zhang, Meng Lensch, Sarah Shields, Cara E Livingston, Mark Weiss, Ron Zhao, Huimin Haynes, Karmella A Morsut, Leonardo Chen, Yvonne Y Khalil, Ahmad S Wong, Wilson W Collins, James J Rosser, Susan J Polizzi, Karen Elowitz, Michael B Fussenegger, Martin Hilton, Isaac B Leonard, Joshua N Bintu, Lacramioara Galloway, Kate E Deans, Tara L Massachusetts Institute of Technology. Department of Biological Engineering To elucidate principles operating in native biological systems and to develop novel biotechnologies, synthetic biology aims to build and integrate synthetic gene circuits within native transcriptional networks. The utility of synthetic gene circuits for cell engineering relies on the ability to control the expression of all constituent transgene components. Transgene silencing, defined as the loss of expression over time, persists as an obstacle for engineering primary cells and stem cells with transgenic cargos. In this review, we highlight the challenge that transgene silencing poses to the robust engineering of mammalian cells, outline potential molecular mechanisms of silencing, and present approaches for preventing transgene silencing. We conclude with a perspective identifying future research directions for improving the performance of synthetic gene circuits. 2023-01-30T19:56:46Z 2023-01-30T19:56:46Z 2022-12 2023-01-30T19:48:43Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/147792 Cabrera, Alan, Edelstein, Hailey I, Glykofrydis, Fokion, Love, Kasey S, Palacios, Sebastian et al. 2022. "The sound of silence: Transgene silencing in mammalian cell engineering." Cell Systems, 13 (12). en 10.1016/j.cels.2022.11.005 Cell Systems Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier BV Elsevier |
| spellingShingle | Cabrera, Alan Edelstein, Hailey I Glykofrydis, Fokion Love, Kasey S Palacios, Sebastian Tycko, Josh Zhang, Meng Lensch, Sarah Shields, Cara E Livingston, Mark Weiss, Ron Zhao, Huimin Haynes, Karmella A Morsut, Leonardo Chen, Yvonne Y Khalil, Ahmad S Wong, Wilson W Collins, James J Rosser, Susan J Polizzi, Karen Elowitz, Michael B Fussenegger, Martin Hilton, Isaac B Leonard, Joshua N Bintu, Lacramioara Galloway, Kate E Deans, Tara L The sound of silence: Transgene silencing in mammalian cell engineering |
| title | The sound of silence: Transgene silencing in mammalian cell engineering |
| title_full | The sound of silence: Transgene silencing in mammalian cell engineering |
| title_fullStr | The sound of silence: Transgene silencing in mammalian cell engineering |
| title_full_unstemmed | The sound of silence: Transgene silencing in mammalian cell engineering |
| title_short | The sound of silence: Transgene silencing in mammalian cell engineering |
| title_sort | sound of silence transgene silencing in mammalian cell engineering |
| url | https://hdl.handle.net/1721.1/147792 |
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