Quantitative mapping of the cellular small RNA landscape with AQRNA-seq

Current next-generation RNA-sequencing (RNA-seq) methods do not provide accurate quantification of small RNAs within a sample, due to sequence-dependent biases in capture, ligation and amplification during library preparation. We present a method, absolute quantification RNA-sequencing (AQRNA-seq),...

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Bibliographic Details
Main Authors: Hu, Jennifer F, Yim, Daniel, Ma, Duanduan, Huber, Sabrina M, Davis, Nick, Bacusmo, Jo Marie, Vermeulen, Sidney, Zhou, Jieliang, Begley, Thomas J, DeMott, Michael S, Levine, Stuart S, de Crécy-Lagard, Valérie, Dedon, Peter C, Cao, Bo
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering
Format: Article
Language:English
Published: Springer Science and Business Media LLC 2023
Online Access:https://hdl.handle.net/1721.1/147800