A Protocol for Studying Transcription Factor Dynamics Using Fast Single-Particle Tracking and Spot-On Model-Based Analysis
Single-particle tracking (SPT) makes it possible to directly observe single protein diffusion dynamics in living cells over time. Thus, SPT has emerged as a powerful method to quantify the dynamics of nuclear proteins such as transcription factors (TFs). Here, we provide a protocol for conducting an...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Springer US
2023
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| Online Access: | https://hdl.handle.net/1721.1/147985 |