Nanozyme-catalysed CRISPR assay for preamplification-free detection of non-coding RNAs
CRISPR-based diagnostics enable specific sensing of DNA and RNA biomarkers associated with human diseases. This is achieved through the binding of guide RNAs to a complementary sequence that activates Cas enzymes to cleave reporter molecules. Currently, most CRISPR-based diagnostics rely on target p...
| Main Authors: | , , , , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Springer Science and Business Media LLC
2023
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| Online Access: | https://hdl.handle.net/1721.1/147986 |
| _version_ | 1826209453479821312 |
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| author | Broto, Marta Kaminski, Michael M Adrianus, Christopher Kim, Nayoung Greensmith, Robert Dissanayake-Perera, Schan Schubert, Alexander J Tan, Xiao Kim, Hyemin Dighe, Anand S Collins, James J Stevens, Molly M |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Broto, Marta Kaminski, Michael M Adrianus, Christopher Kim, Nayoung Greensmith, Robert Dissanayake-Perera, Schan Schubert, Alexander J Tan, Xiao Kim, Hyemin Dighe, Anand S Collins, James J Stevens, Molly M |
| author_sort | Broto, Marta |
| collection | MIT |
| description | CRISPR-based diagnostics enable specific sensing of DNA and RNA biomarkers associated with human diseases. This is achieved through the binding of guide RNAs to a complementary sequence that activates Cas enzymes to cleave reporter molecules. Currently, most CRISPR-based diagnostics rely on target preamplification to reach sufficient sensitivity for clinical applications. This limits quantification capability and adds complexity to the reaction chemistry. Here we show the combination of a CRISPR-Cas-based reaction with a nanozyme-linked immunosorbent assay, which allows for the quantitative and colorimetric readout of Cas13-mediated RNA detection through catalytic metallic nanoparticles at room temperature (CrisprZyme). We demonstrate that CrisprZyme is easily adaptable to a lateral-flow-based readout and different Cas enzymes and enables the sensing of non-coding RNAs including microRNAs, long non-coding RNAs and circular RNAs. We utilize this platform to identify patients with acute myocardial infarction and to monitor cellular differentiation in vitro and in tissue biopsies from prostate cancer patients. We anticipate that CrisprZyme will serve as a universally applicable signal catalyst for CRISPR-based diagnostics, which will expand the spectrum of targets for preamplification-free, quantitative detection. |
| first_indexed | 2024-09-23T14:22:45Z |
| format | Article |
| id | mit-1721.1/147986 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T14:22:45Z |
| publishDate | 2023 |
| publisher | Springer Science and Business Media LLC |
| record_format | dspace |
| spelling | mit-1721.1/1479862023-02-10T03:15:51Z Nanozyme-catalysed CRISPR assay for preamplification-free detection of non-coding RNAs Broto, Marta Kaminski, Michael M Adrianus, Christopher Kim, Nayoung Greensmith, Robert Dissanayake-Perera, Schan Schubert, Alexander J Tan, Xiao Kim, Hyemin Dighe, Anand S Collins, James J Stevens, Molly M Massachusetts Institute of Technology. Department of Biological Engineering CRISPR-based diagnostics enable specific sensing of DNA and RNA biomarkers associated with human diseases. This is achieved through the binding of guide RNAs to a complementary sequence that activates Cas enzymes to cleave reporter molecules. Currently, most CRISPR-based diagnostics rely on target preamplification to reach sufficient sensitivity for clinical applications. This limits quantification capability and adds complexity to the reaction chemistry. Here we show the combination of a CRISPR-Cas-based reaction with a nanozyme-linked immunosorbent assay, which allows for the quantitative and colorimetric readout of Cas13-mediated RNA detection through catalytic metallic nanoparticles at room temperature (CrisprZyme). We demonstrate that CrisprZyme is easily adaptable to a lateral-flow-based readout and different Cas enzymes and enables the sensing of non-coding RNAs including microRNAs, long non-coding RNAs and circular RNAs. We utilize this platform to identify patients with acute myocardial infarction and to monitor cellular differentiation in vitro and in tissue biopsies from prostate cancer patients. We anticipate that CrisprZyme will serve as a universally applicable signal catalyst for CRISPR-based diagnostics, which will expand the spectrum of targets for preamplification-free, quantitative detection. 2023-02-09T14:29:23Z 2023-02-09T14:29:23Z 2022 2023-02-09T14:17:02Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/147986 Broto, Marta, Kaminski, Michael M, Adrianus, Christopher, Kim, Nayoung, Greensmith, Robert et al. 2022. "Nanozyme-catalysed CRISPR assay for preamplification-free detection of non-coding RNAs." Nature Nanotechnology, 17 (10). en 10.1038/S41565-022-01179-0 Nature Nanotechnology Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Springer Science and Business Media LLC Prof. Collins |
| spellingShingle | Broto, Marta Kaminski, Michael M Adrianus, Christopher Kim, Nayoung Greensmith, Robert Dissanayake-Perera, Schan Schubert, Alexander J Tan, Xiao Kim, Hyemin Dighe, Anand S Collins, James J Stevens, Molly M Nanozyme-catalysed CRISPR assay for preamplification-free detection of non-coding RNAs |
| title | Nanozyme-catalysed CRISPR assay for preamplification-free detection of non-coding RNAs |
| title_full | Nanozyme-catalysed CRISPR assay for preamplification-free detection of non-coding RNAs |
| title_fullStr | Nanozyme-catalysed CRISPR assay for preamplification-free detection of non-coding RNAs |
| title_full_unstemmed | Nanozyme-catalysed CRISPR assay for preamplification-free detection of non-coding RNAs |
| title_short | Nanozyme-catalysed CRISPR assay for preamplification-free detection of non-coding RNAs |
| title_sort | nanozyme catalysed crispr assay for preamplification free detection of non coding rnas |
| url | https://hdl.handle.net/1721.1/147986 |
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