Yeast Display for the Identification of Peptide-MHC Ligands of Immune Receptors
T cells detect peptide antigens presented by major histocompatibility complex (MHC) proteins via their T cell receptor (TCR). The sequence diversity of possible antigens, with trillions of potential peptide-MHC targets, makes it challenging to study, characterize, and manipulate the peptide repertoi...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Springer US
2023
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| Online Access: | https://hdl.handle.net/1721.1/148130 |
| _version_ | 1811083185803493376 |
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| author | Huisman, Brooke D Grace, Beth E Holec, Patrick V Birnbaum, Michael E |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Huisman, Brooke D Grace, Beth E Holec, Patrick V Birnbaum, Michael E |
| author_sort | Huisman, Brooke D |
| collection | MIT |
| description | T cells detect peptide antigens presented by major histocompatibility complex (MHC) proteins via their T cell receptor (TCR). The sequence diversity of possible antigens, with trillions of potential peptide-MHC targets, makes it challenging to study, characterize, and manipulate the peptide repertoire of a given TCR. Yeast display has been utilized to study the interactions between peptide-MHCs and T cell receptors to facilitate high-throughput screening of peptide-MHC libraries. Here we present insights on designing and validating a peptide-MHC yeast display construct, designing and constructing peptide libraries, conducting selections, and preparing, processing, and analyzing peptide library sequencing data. Applications for this approach are broad, including characterizing peptide-MHC recognition profiles for a TCR, screening for high-affinity mimotopes of known TCR-binding peptides, and identifying natural ligands of TCRs from expanded T cells. |
| first_indexed | 2024-09-23T12:27:34Z |
| format | Article |
| id | mit-1721.1/148130 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T12:27:34Z |
| publishDate | 2023 |
| publisher | Springer US |
| record_format | dspace |
| spelling | mit-1721.1/1481302023-05-08T04:22:52Z Yeast Display for the Identification of Peptide-MHC Ligands of Immune Receptors Huisman, Brooke D Grace, Beth E Holec, Patrick V Birnbaum, Michael E Massachusetts Institute of Technology. Department of Biological Engineering T cells detect peptide antigens presented by major histocompatibility complex (MHC) proteins via their T cell receptor (TCR). The sequence diversity of possible antigens, with trillions of potential peptide-MHC targets, makes it challenging to study, characterize, and manipulate the peptide repertoire of a given TCR. Yeast display has been utilized to study the interactions between peptide-MHCs and T cell receptors to facilitate high-throughput screening of peptide-MHC libraries. Here we present insights on designing and validating a peptide-MHC yeast display construct, designing and constructing peptide libraries, conducting selections, and preparing, processing, and analyzing peptide library sequencing data. Applications for this approach are broad, including characterizing peptide-MHC recognition profiles for a TCR, screening for high-affinity mimotopes of known TCR-binding peptides, and identifying natural ligands of TCRs from expanded T cells. 2023-02-21T18:10:26Z 2023-02-21T18:10:26Z 2022 2023-02-21T18:03:23Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/148130 Huisman, Brooke D, Grace, Beth E, Holec, Patrick V and Birnbaum, Michael E. 2022. "Yeast Display for the Identification of Peptide-MHC Ligands of Immune Receptors." Methods Mol Biol, 2491. en 10.1007/978-1-0716-2285-8_15 Methods Mol Biol Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf application/vnd.openxmlformats-officedocument.presentationml.presentation Springer US Prof. Birnbaum |
| spellingShingle | Huisman, Brooke D Grace, Beth E Holec, Patrick V Birnbaum, Michael E Yeast Display for the Identification of Peptide-MHC Ligands of Immune Receptors |
| title | Yeast Display for the Identification of Peptide-MHC Ligands of Immune Receptors |
| title_full | Yeast Display for the Identification of Peptide-MHC Ligands of Immune Receptors |
| title_fullStr | Yeast Display for the Identification of Peptide-MHC Ligands of Immune Receptors |
| title_full_unstemmed | Yeast Display for the Identification of Peptide-MHC Ligands of Immune Receptors |
| title_short | Yeast Display for the Identification of Peptide-MHC Ligands of Immune Receptors |
| title_sort | yeast display for the identification of peptide mhc ligands of immune receptors |
| url | https://hdl.handle.net/1721.1/148130 |
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