Rapid directed molecular evolution of fluorescent proteins in mammalian cells

Rapid directed molecular evolution of fluorescent proteins in mammalian cells

In vivo imaging of model organisms is heavily reliant on fluorescent proteins with high intracellular brightness. Here we describe a practical method for rapid optimization of fluorescent proteins via directed molecular evolution in cultured mammalian cells. Using this method, we were able to perfor...

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Bibliographic Details
Main Authors: Babakhanova, Siranush, Jung, Erica E, Namikawa, Kazuhiko, Zhang, Hanbin, Wang, Yangdong, Subach, Oksana M, Korzhenevskiy, Dmitry A, Rakitina, Tatiana V, Xiao, Xian, Wang, Wenjing, Shi, Jing, Drobizhev, Mikhail, Park, Demian, Eisenhard, Lea, Tang, Hongyun, Köster, Reinhard W, Subach, Fedor V, Boyden, Edward S, Piatkevich, Kiryl D
Other Authors: Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Format: Article
Language:English
Published: Wiley 2023
Online Access:https://hdl.handle.net/1721.1/148695

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https://hdl.handle.net/1721.1/148695

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