Effects of Storage Temperatures on Nitrogen Assimilation and Remobilization during Post-Harvest Senescence of Pak Choi
In the agricultural industry, the post-harvest leafy vegetable quality and shelf life significantly influence market value and consumer acceptability. This study examined the effects of different storage temperatures on leaf senescence, nitrogen assimilation, and remobilization in Pak Choi (<i>...
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| Format: | Article |
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Multidisciplinary Digital Publishing Institute
2023
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| Online Access: | https://hdl.handle.net/1721.1/152538 |
| _version_ | 1811075645595189248 |
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| author | Dhandapani, Savitha Philip, Vidya Susan Nabeela Nasreen, Shaik Anwar Ahamed Tan, Alice Mei Xien Jayapal, Praveen Kumar Ram, Rajeev J. Park, Bong Soo |
| author2 | Singapore-MIT Alliance in Research and Technology (SMART) |
| author_facet | Singapore-MIT Alliance in Research and Technology (SMART) Dhandapani, Savitha Philip, Vidya Susan Nabeela Nasreen, Shaik Anwar Ahamed Tan, Alice Mei Xien Jayapal, Praveen Kumar Ram, Rajeev J. Park, Bong Soo |
| author_sort | Dhandapani, Savitha |
| collection | MIT |
| description | In the agricultural industry, the post-harvest leafy vegetable quality and shelf life significantly influence market value and consumer acceptability. This study examined the effects of different storage temperatures on leaf senescence, nitrogen assimilation, and remobilization in Pak Choi (<i>Brassica rapa</i> subsp. <i>chinensis</i>). Mature Pak Choi plants were harvested and stored at two different temperatures, 4 °C and 25 °C. Senescence was tracked via chlorophyll content and leaf yellowing. Concurrently, alterations in the total nitrogen, nitrate, and protein content were quantified on days 0, 3, 6, and 9 in old, mid, and young leaves of Pak Choi plants. As expected, 4 °C alleviated chlorophyll degradation and delayed senescence of Pak Choi compared to 25 °C. Total nitrogen and protein contents were inversely correlated, while the nitrate content remained nearly constant across leaf groups at 25 °C. Additionally, the transcript levels of genes involved in nitrogen assimilation and remobilization revealed key candidate genes that were differentially expressed between 4 °C and 25 °C, which might be targeted to extend the shelf life of the leafy vegetables. Thus, this study provides pivotal insights into the molecular and physiological responses of Pak Choi to post-harvest storage conditions. |
| first_indexed | 2024-09-23T10:09:43Z |
| format | Article |
| id | mit-1721.1/152538 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T10:09:43Z |
| publishDate | 2023 |
| publisher | Multidisciplinary Digital Publishing Institute |
| record_format | dspace |
| spelling | mit-1721.1/1525382024-01-22T21:47:38Z Effects of Storage Temperatures on Nitrogen Assimilation and Remobilization during Post-Harvest Senescence of Pak Choi Dhandapani, Savitha Philip, Vidya Susan Nabeela Nasreen, Shaik Anwar Ahamed Tan, Alice Mei Xien Jayapal, Praveen Kumar Ram, Rajeev J. Park, Bong Soo Singapore-MIT Alliance in Research and Technology (SMART) Massachusetts Institute of Technology. Research Laboratory of Electronics In the agricultural industry, the post-harvest leafy vegetable quality and shelf life significantly influence market value and consumer acceptability. This study examined the effects of different storage temperatures on leaf senescence, nitrogen assimilation, and remobilization in Pak Choi (<i>Brassica rapa</i> subsp. <i>chinensis</i>). Mature Pak Choi plants were harvested and stored at two different temperatures, 4 °C and 25 °C. Senescence was tracked via chlorophyll content and leaf yellowing. Concurrently, alterations in the total nitrogen, nitrate, and protein content were quantified on days 0, 3, 6, and 9 in old, mid, and young leaves of Pak Choi plants. As expected, 4 °C alleviated chlorophyll degradation and delayed senescence of Pak Choi compared to 25 °C. Total nitrogen and protein contents were inversely correlated, while the nitrate content remained nearly constant across leaf groups at 25 °C. Additionally, the transcript levels of genes involved in nitrogen assimilation and remobilization revealed key candidate genes that were differentially expressed between 4 °C and 25 °C, which might be targeted to extend the shelf life of the leafy vegetables. Thus, this study provides pivotal insights into the molecular and physiological responses of Pak Choi to post-harvest storage conditions. 2023-10-27T19:51:47Z 2023-10-27T19:51:47Z 2023-10-18 2023-10-27T10:27:07Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/152538 Biomolecules 13 (10): 1540 (2023) PUBLISHER_CC http://dx.doi.org/10.3390/biom13101540 Creative Commons Attribution https://creativecommons.org/licenses/by/4.0/ application/pdf Multidisciplinary Digital Publishing Institute Multidisciplinary Digital Publishing Institute |
| spellingShingle | Dhandapani, Savitha Philip, Vidya Susan Nabeela Nasreen, Shaik Anwar Ahamed Tan, Alice Mei Xien Jayapal, Praveen Kumar Ram, Rajeev J. Park, Bong Soo Effects of Storage Temperatures on Nitrogen Assimilation and Remobilization during Post-Harvest Senescence of Pak Choi |
| title | Effects of Storage Temperatures on Nitrogen Assimilation and Remobilization during Post-Harvest Senescence of Pak Choi |
| title_full | Effects of Storage Temperatures on Nitrogen Assimilation and Remobilization during Post-Harvest Senescence of Pak Choi |
| title_fullStr | Effects of Storage Temperatures on Nitrogen Assimilation and Remobilization during Post-Harvest Senescence of Pak Choi |
| title_full_unstemmed | Effects of Storage Temperatures on Nitrogen Assimilation and Remobilization during Post-Harvest Senescence of Pak Choi |
| title_short | Effects of Storage Temperatures on Nitrogen Assimilation and Remobilization during Post-Harvest Senescence of Pak Choi |
| title_sort | effects of storage temperatures on nitrogen assimilation and remobilization during post harvest senescence of pak choi |
| url | https://hdl.handle.net/1721.1/152538 |
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