CRISPR-Cas9 knockout screen informs efficient reduction of the Komagataella phaffii secretome

CRISPR-Cas9 knockout screen informs efficient reduction of the Komagataella phaffii secretome

Background The yeast Komagataella phaffii is widely used for manufacturing recombinant proteins, but secreted titers of recombinant proteins could be improved by genetic engineering. In this study, we hypothesized that cellular resources could be redirected from production of endogenous proteins to...

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Bibliographic Details
Main Authors: Dalvie, Neil C., Lorgeree, Timothy R., Yang, Yuchen, Rodriguez-Aponte, Sergio A., Whittaker, Charles A., Hinckley, Joshua A., Clark, John J., Del Rosario, Amanda M., Love, Kerry R., Love, J. C.
Format: Article
Language:English
Published: BioMed Central 2024
Online Access:https://hdl.handle.net/1721.1/155951

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https://hdl.handle.net/1721.1/155951

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