Cluster-selective 57Fe labeling of a Twitch-domaincontaining radical SAM enzyme
57Fe-specific techniques such as Mössbauer spectroscopy are invaluable tools in mechanistic studies of Fe–S proteins. However, they remain underutilized for proteins that bind multiple Fe–S clusters because such proteins are typically uniformly enriched with 57Fe. As a result, it can be unclear whic...
Main Authors: | , |
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Other Authors: | |
Format: | Article |
Language: | English |
Published: |
Royal Society of Chemistry
2024
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Online Access: | https://hdl.handle.net/1721.1/156920 |