Multidose transient transfection of human embryonic kidney 293 cells modulates recombinant adeno‐associated virus2/5 Rep protein expression and influences the enrichment fraction of filled capsids

Multidose transient transfection of human embryonic kidney 293 cells modulates recombinant adeno‐associated virus2/5 Rep protein expression and influences the enrichment fraction of filled capsids

Recombinant adeno-associated virus (rAAV) is a commonly used in vivo gene therapy vector because of its nonpathogenicity, long-term transgene expression, broad tropism, and ability to transduce both dividing and nondividing cells. However, rAAV vector production via transient transfection of mammali...

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Bibliographic Details
Main Authors: Srinivasan, Prasanna, Canova, Christopher T, Sha, Sha, Nguyen, Tam NT, Joseph, John, Sangerman, Jose, Maloney, Andrew J, Katsikis, Georgios, Ou, Rui Wen, Hong, Moo Sun, Ng, Jaclyn, Yuan, Arella, Antov, Daniel, Song, Sally, Chen, Wenyu, Neufeld, Caleb, Wolfrum, Jacqueline M, Barone, Paul W, Sinskey, Anthony J, Springs, Stacy L, Braatz, Richard D
Other Authors: Massachusetts Institute of Technology. Center for Biomedical Innovation
Format: Article
Language:English
Published: Wiley 2024
Online Access:https://hdl.handle.net/1721.1/157695

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https://hdl.handle.net/1721.1/157695

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