High-Level Expression, Single-Step Immunoaffinity Purification and Characterization of Human Tetraspanin Membrane Protein CD81

High-Level Expression, Single-Step Immunoaffinity Purification and Characterization of Human Tetraspanin Membrane Protein CD81

The study of membrane protein structure and function requires their high-level expression and purification in fully functional form. We previously used a tetracycline-inducible stable mammalian cell line, HEK293S-TetR, for regulated high-level expression of G-protein coupled receptors. We here repor...

Full description

Bibliographic Details
Main Authors: Khorana, H. Gobind, Zhang, Shuguang, Chelikani, Prashen, Takayama, Hidehito, Reeves, Philip J.
Other Authors: Massachusetts Institute of Technology. Center for Biomedical Engineering
Format: Article
Language:en_US
Published: Public Library of Science 2010
Online Access:http://hdl.handle.net/1721.1/55362

Internet

http://hdl.handle.net/1721.1/55362

Similar Items