Dynamic association of the replication initiator and transcription factor DnaA with the Bacillus subtilis chromosome during replication stress
DnaA functions as both a transcription factor and the replication initiator in bacteria. We characterized the DNA binding dynamics of DnaA on a genomic level. Based on cross-linking and chromatin immunoprecipitation data, DnaA binds at least 17 loci, 15 of which are regulated transcriptionally in re...
| Main Authors: | , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
American Society for Microbiology
2010
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/1721.1/57484 https://orcid.org/0000-0002-8235-7227 |
| _version_ | 1811074588715515904 |
|---|---|
| author | Breier, Adam M. Grossman, Alan Davis |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Breier, Adam M. Grossman, Alan Davis |
| author_sort | Breier, Adam M. |
| collection | MIT |
| description | DnaA functions as both a transcription factor and the replication initiator in bacteria. We characterized the DNA binding dynamics of DnaA on a genomic level. Based on cross-linking and chromatin immunoprecipitation data, DnaA binds at least 17 loci, 15 of which are regulated transcriptionally in response to inhibition of replication (replication stress). Six loci, each of which has a cluster of at least nine potential DnaA binding sites, had significant increases in binding by DnaA when replication was inhibited, indicating that the association of DnaA with at least some of its target sites is altered after replication stress. When replication resumed from oriC after inhibition of replication initiation, these high levels of binding decreased rapidly at origin-proximal and origin-distal regions, well before a replication fork could pass through each of the regulated regions. These findings indicate that there is rapid signaling to decrease activation of DnaA during replication and that interaction between DnaA bound at each site and the replication machinery is not required for regulation of DnaA activity in response to replication stress. |
| first_indexed | 2024-09-23T09:52:12Z |
| format | Article |
| id | mit-1721.1/57484 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:52:12Z |
| publishDate | 2010 |
| publisher | American Society for Microbiology |
| record_format | dspace |
| spelling | mit-1721.1/574842022-09-30T17:20:44Z Dynamic association of the replication initiator and transcription factor DnaA with the Bacillus subtilis chromosome during replication stress Breier, Adam M. Grossman, Alan Davis Massachusetts Institute of Technology. Department of Biology Grossman, Alan D. Grossman, Alan D. Breier, Adam M. Bacillus subtilis DnaA replication stress transcription DnaA functions as both a transcription factor and the replication initiator in bacteria. We characterized the DNA binding dynamics of DnaA on a genomic level. Based on cross-linking and chromatin immunoprecipitation data, DnaA binds at least 17 loci, 15 of which are regulated transcriptionally in response to inhibition of replication (replication stress). Six loci, each of which has a cluster of at least nine potential DnaA binding sites, had significant increases in binding by DnaA when replication was inhibited, indicating that the association of DnaA with at least some of its target sites is altered after replication stress. When replication resumed from oriC after inhibition of replication initiation, these high levels of binding decreased rapidly at origin-proximal and origin-distal regions, well before a replication fork could pass through each of the regulated regions. These findings indicate that there is rapid signaling to decrease activation of DnaA during replication and that interaction between DnaA bound at each site and the replication machinery is not required for regulation of DnaA activity in response to replication stress. 2010-08-10T18:27:21Z 2010-08-10T18:27:21Z 2009-01 2008-09 Article http://purl.org/eprint/type/SubmittedJournalArticle 0021-9193 http://hdl.handle.net/1721.1/57484 Breier, Adam M., Grossman, Alan D. Dynamic Association of the Replication Initiator and Transcription Factor DnaA with the Bacillus subtilis Chromosome during Replication Stress J. Bacteriol. 2009 191: 486-493 19011033 https://orcid.org/0000-0002-8235-7227 en_US http://dx.doi.org/10.1128/jb.01294-08 Journal of Bacteriology Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Society for Microbiology Alan Grossman |
| spellingShingle | Bacillus subtilis DnaA replication stress transcription Breier, Adam M. Grossman, Alan Davis Dynamic association of the replication initiator and transcription factor DnaA with the Bacillus subtilis chromosome during replication stress |
| title | Dynamic association of the replication initiator and transcription factor DnaA with the Bacillus subtilis chromosome during replication stress |
| title_full | Dynamic association of the replication initiator and transcription factor DnaA with the Bacillus subtilis chromosome during replication stress |
| title_fullStr | Dynamic association of the replication initiator and transcription factor DnaA with the Bacillus subtilis chromosome during replication stress |
| title_full_unstemmed | Dynamic association of the replication initiator and transcription factor DnaA with the Bacillus subtilis chromosome during replication stress |
| title_short | Dynamic association of the replication initiator and transcription factor DnaA with the Bacillus subtilis chromosome during replication stress |
| title_sort | dynamic association of the replication initiator and transcription factor dnaa with the bacillus subtilis chromosome during replication stress |
| topic | Bacillus subtilis DnaA replication stress transcription |
| url | http://hdl.handle.net/1721.1/57484 https://orcid.org/0000-0002-8235-7227 |
| work_keys_str_mv | AT breieradamm dynamicassociationofthereplicationinitiatorandtranscriptionfactordnaawiththebacillussubtilischromosomeduringreplicationstress AT grossmanalandavis dynamicassociationofthereplicationinitiatorandtranscriptionfactordnaawiththebacillussubtilischromosomeduringreplicationstress |