Degradation of Postsynaptic Scaffold GKAP and Regulation of Dendritic Spine Morphology by the TRIM[subscript 3] Ubiquitin Ligase in Rat Hippocampal Neurons
Changes in neuronal activity modify the structure of dendritic spines and alter the function and protein composition of synapses. Regulated degradation of postsynaptic density (PSD) proteins by the ubiquitin-proteasome system is believed to play an important role in activity-dependent synaptic remod...
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| Format: | Article |
| Language: | en_US |
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Public Library of Science
2010
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| Online Access: | http://hdl.handle.net/1721.1/57572 |
| _version_ | 1811089809517576192 |
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| author | Sheng, Morgan Hwa-Tze Sung, Clifford C. Brito, Ilana Lauren Hung, Albert Y. |
| author2 | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences |
| author_facet | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Sheng, Morgan Hwa-Tze Sung, Clifford C. Brito, Ilana Lauren Hung, Albert Y. |
| author_sort | Sheng, Morgan Hwa-Tze |
| collection | MIT |
| description | Changes in neuronal activity modify the structure of dendritic spines and alter the function and protein composition of synapses. Regulated degradation of postsynaptic density (PSD) proteins by the ubiquitin-proteasome system is believed to play an important role in activity-dependent synaptic remodeling. Stimulating neuronal activity in vitro and in vivo induces the ubiquitination and degradation of GKAP/SAPAP and Shank, major scaffold proteins of the PSD. However, the specific ubiquitin ligases that regulate postsynaptic protein composition have not been identified. Here we identify the RING finger-containing protein TRIM3 as a specific E3 ubiquitin ligase for the PSD scaffold GKAP/SAPAP1. Present in PSD fractions from rat brain, TRIM3 stimulates ubiquitination and proteasome-dependent degradation of GKAP, and induces the loss of GKAP and associated scaffold Shank1 from postsynaptic sites. Suppression of endogenous TRIM3 by RNA interference (RNAi) results in increased accumulation of GKAP and Shank1 at synapses, as well as enlargement of dendritic spine heads. RNAi of TRIM3 also prevented the loss of GKAP induced by synaptic activity. Thus, TRIM3 is a novel E3 ligase that mediates activity-dependent turnover of PSD scaffold proteins and is a negative regulator of dendritic spine morphology. |
| first_indexed | 2024-09-23T14:25:01Z |
| format | Article |
| id | mit-1721.1/57572 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:25:01Z |
| publishDate | 2010 |
| publisher | Public Library of Science |
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| spelling | mit-1721.1/575722022-09-29T09:21:40Z Degradation of Postsynaptic Scaffold GKAP and Regulation of Dendritic Spine Morphology by the TRIM[subscript 3] Ubiquitin Ligase in Rat Hippocampal Neurons Degradation of Postsynaptic Scaffold GKAP and Regulation of Dendritic Spine Morphology by the TRIM3 Ubiquitin Ligase in Rat Hippocampal Neurons Sheng, Morgan Hwa-Tze Sung, Clifford C. Brito, Ilana Lauren Hung, Albert Y. Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Picower Institute for Learning and Memory Sheng, Morgan Hwa-Tze Sheng, Morgan Hwa-Tze Sung, Clifford C. Brito, Ilana Lauren Hung, Albert Y. Changes in neuronal activity modify the structure of dendritic spines and alter the function and protein composition of synapses. Regulated degradation of postsynaptic density (PSD) proteins by the ubiquitin-proteasome system is believed to play an important role in activity-dependent synaptic remodeling. Stimulating neuronal activity in vitro and in vivo induces the ubiquitination and degradation of GKAP/SAPAP and Shank, major scaffold proteins of the PSD. However, the specific ubiquitin ligases that regulate postsynaptic protein composition have not been identified. Here we identify the RING finger-containing protein TRIM3 as a specific E3 ubiquitin ligase for the PSD scaffold GKAP/SAPAP1. Present in PSD fractions from rat brain, TRIM3 stimulates ubiquitination and proteasome-dependent degradation of GKAP, and induces the loss of GKAP and associated scaffold Shank1 from postsynaptic sites. Suppression of endogenous TRIM3 by RNA interference (RNAi) results in increased accumulation of GKAP and Shank1 at synapses, as well as enlargement of dendritic spine heads. RNAi of TRIM3 also prevented the loss of GKAP induced by synaptic activity. Thus, TRIM3 is a novel E3 ligase that mediates activity-dependent turnover of PSD scaffold proteins and is a negative regulator of dendritic spine morphology. 2010-08-26T16:14:10Z 2010-08-26T16:14:10Z 2010-03 2009-09 Article http://purl.org/eprint/type/JournalArticle 1932-6203 http://hdl.handle.net/1721.1/57572 Hung, Albert Y. et al. “Degradation of Postsynaptic Scaffold GKAP and Regulation of Dendritic Spine Morphology by the TRIM3 Ubiquitin Ligase in Rat Hippocampal Neurons.” PLoS ONE 5.3 (2010): e9842. en_US http://dx.doi.org/10.1371/journal.pone.0009842 PLoS ONE Creative Commons Attribution http://creativecommons.org/licenses/by/2.5/ application/pdf Public Library of Science PLoS |
| spellingShingle | Sheng, Morgan Hwa-Tze Sung, Clifford C. Brito, Ilana Lauren Hung, Albert Y. Degradation of Postsynaptic Scaffold GKAP and Regulation of Dendritic Spine Morphology by the TRIM[subscript 3] Ubiquitin Ligase in Rat Hippocampal Neurons |
| title | Degradation of Postsynaptic Scaffold GKAP and Regulation of Dendritic Spine Morphology by the TRIM[subscript 3] Ubiquitin Ligase in Rat Hippocampal Neurons |
| title_full | Degradation of Postsynaptic Scaffold GKAP and Regulation of Dendritic Spine Morphology by the TRIM[subscript 3] Ubiquitin Ligase in Rat Hippocampal Neurons |
| title_fullStr | Degradation of Postsynaptic Scaffold GKAP and Regulation of Dendritic Spine Morphology by the TRIM[subscript 3] Ubiquitin Ligase in Rat Hippocampal Neurons |
| title_full_unstemmed | Degradation of Postsynaptic Scaffold GKAP and Regulation of Dendritic Spine Morphology by the TRIM[subscript 3] Ubiquitin Ligase in Rat Hippocampal Neurons |
| title_short | Degradation of Postsynaptic Scaffold GKAP and Regulation of Dendritic Spine Morphology by the TRIM[subscript 3] Ubiquitin Ligase in Rat Hippocampal Neurons |
| title_sort | degradation of postsynaptic scaffold gkap and regulation of dendritic spine morphology by the trim subscript 3 ubiquitin ligase in rat hippocampal neurons |
| url | http://hdl.handle.net/1721.1/57572 |
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