Microfluidic Concentration-Enhanced Cellular Kinase Activity Assay

In this paper, we reported a simple, disposable PDMS micro/nanofluidic preconcentration chip for in vitro concentration-enhanced cell kinase assays. Utilizing the preconcentration (electrokinetic trapping) directly from cell lysate (1 mM ATP) samples, we could achieve at least a 25-fold increase in...

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Bibliographic Details
Main Authors: Lee, Jeong Hoon, Cosgrove, Benjamin D., Lauffenburger, Douglas A., Han, Jongyoon
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering
Format: Article
Language:en_US
Published: American Chemical Society 2010
Online Access:http://hdl.handle.net/1721.1/60317