Microfluidic Concentration-Enhanced Cellular Kinase Activity Assay
In this paper, we reported a simple, disposable PDMS micro/nanofluidic preconcentration chip for in vitro concentration-enhanced cell kinase assays. Utilizing the preconcentration (electrokinetic trapping) directly from cell lysate (1 mM ATP) samples, we could achieve at least a 25-fold increase in...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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American Chemical Society
2010
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| Online Access: | http://hdl.handle.net/1721.1/60317 |
| _version_ | 1826205570233794560 |
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| author | Lee, Jeong Hoon Cosgrove, Benjamin D. Lauffenburger, Douglas A. Han, Jongyoon |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Lee, Jeong Hoon Cosgrove, Benjamin D. Lauffenburger, Douglas A. Han, Jongyoon |
| author_sort | Lee, Jeong Hoon |
| collection | MIT |
| description | In this paper, we reported a simple, disposable PDMS micro/nanofluidic preconcentration chip for in vitro concentration-enhanced cell kinase assays. Utilizing the preconcentration (electrokinetic trapping) directly from cell lysate (1 mM ATP) samples, we could achieve at least a 25-fold increase in reaction velocity and 65-fold enhancement in sensitivity. In addition, we shorten the assay time down to less than 10 min, with the sample volume requirements of down to 5 cells. This device could be a generic and powerful tool for diagnostics and systems biology studies at the single-cell level, if properly optimized and integrated with the cell culture microdevices. |
| first_indexed | 2024-09-23T13:15:32Z |
| format | Article |
| id | mit-1721.1/60317 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:15:32Z |
| publishDate | 2010 |
| publisher | American Chemical Society |
| record_format | dspace |
| spelling | mit-1721.1/603172022-10-01T14:02:27Z Microfluidic Concentration-Enhanced Cellular Kinase Activity Assay Lee, Jeong Hoon Cosgrove, Benjamin D. Lauffenburger, Douglas A. Han, Jongyoon Massachusetts Institute of Technology. Department of Biological Engineering Lauffenburger, Douglas A. Lauffenburger, Douglas A. Cosgrove, Benjamin D. In this paper, we reported a simple, disposable PDMS micro/nanofluidic preconcentration chip for in vitro concentration-enhanced cell kinase assays. Utilizing the preconcentration (electrokinetic trapping) directly from cell lysate (1 mM ATP) samples, we could achieve at least a 25-fold increase in reaction velocity and 65-fold enhancement in sensitivity. In addition, we shorten the assay time down to less than 10 min, with the sample volume requirements of down to 5 cells. This device could be a generic and powerful tool for diagnostics and systems biology studies at the single-cell level, if properly optimized and integrated with the cell culture microdevices. National Institutes of Health (U.S.) (R01-EB005743) National Institutes of Health (U.S.) (R01-CA119402) National Institutes of Health (U.S.) (P50-GM68672) Institute for Collaborative Biotechnologies 2010-12-17T21:26:12Z 2010-12-17T21:26:12Z 2009-07 Article http://purl.org/eprint/type/JournalArticle 0002-7863 1520-5126 http://hdl.handle.net/1721.1/60317 Lee, Jeong Hoon et al. “Microfluidic Concentration-Enhanced Cellular Kinase Activity Assay.” Journal of the American Chemical Society 131.30 (2009): 10340-10341. en_US http://pubs.acs.org/doi/abs/10.1021/ja902594f Journal of the American Chemical Society Attribution-Noncommercial-Share Alike 3.0 Unported http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf American Chemical Society Prof. Lauffenburger |
| spellingShingle | Lee, Jeong Hoon Cosgrove, Benjamin D. Lauffenburger, Douglas A. Han, Jongyoon Microfluidic Concentration-Enhanced Cellular Kinase Activity Assay |
| title | Microfluidic Concentration-Enhanced Cellular Kinase Activity Assay |
| title_full | Microfluidic Concentration-Enhanced Cellular Kinase Activity Assay |
| title_fullStr | Microfluidic Concentration-Enhanced Cellular Kinase Activity Assay |
| title_full_unstemmed | Microfluidic Concentration-Enhanced Cellular Kinase Activity Assay |
| title_short | Microfluidic Concentration-Enhanced Cellular Kinase Activity Assay |
| title_sort | microfluidic concentration enhanced cellular kinase activity assay |
| url | http://hdl.handle.net/1721.1/60317 |
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