A DinB variant reveals diverse physiological consequences of incomplete TLS extension by a Y-family DNA polymerase
The only Y-family DNA polymerase conserved among all domains of life, DinB and its mammalian ortholog pol κ, catalyzes proficient bypass of damaged DNA in translesion synthesis (TLS). Y-family DNA polymerases, including DinB, have been implicated in diverse biological phenomena ranging from adaptive...
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| Format: | Article |
| Language: | en_US |
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National Academy of Sciences (U.S.)
2011
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| Online Access: | http://hdl.handle.net/1721.1/61367 https://orcid.org/0000-0001-6159-0778 https://orcid.org/0000-0001-7243-8261 https://orcid.org/0000-0002-2196-5691 |
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| author | Walker, Graham C. Jarosz, Daniel F. Delaney, James C. Essigmann, John M. Cohen, Susan E., Ph. D. Massachusetts Institute of Technology |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Walker, Graham C. Jarosz, Daniel F. Delaney, James C. Essigmann, John M. Cohen, Susan E., Ph. D. Massachusetts Institute of Technology |
| author_sort | Walker, Graham C. |
| collection | MIT |
| description | The only Y-family DNA polymerase conserved among all domains of life, DinB and its mammalian ortholog pol κ, catalyzes proficient bypass of damaged DNA in translesion synthesis (TLS). Y-family DNA polymerases, including DinB, have been implicated in diverse biological phenomena ranging from adaptive mutagenesis in bacteria to several human cancers. Complete TLS requires dNTP insertion opposite a replication blocking lesion and subsequent extension with several dNTP additions. Here we report remarkably proficient TLS extension by DinB from Escherichia coli. We also describe a TLS DNA polymerase variant generated by mutation of an evolutionarily conserved tyrosine (Y79). This mutant DinB protein is capable of catalyzing dNTP insertion opposite a replication-blocking lesion, but cannot complete TLS, stalling three nucleotides after an N2[superscript2]-dG adduct. Strikingly, expression of this variant transforms a bacteriostatic DNA damaging agent into a bactericidal drug, resulting in profound toxicity even in a dinB+ background. We find that this phenomenon is not exclusively due to a futile cycle of abortive TLS followed by exonucleolytic reversal. Rather, gene products with roles in cell death and metal homeostasis modulate the toxicity of DinB(Y79L) expression. Together, these results indicate that DinB is specialized to perform remarkably proficient insertion and extension on damaged DNA, and also expose unexpected connections between TLS and cell fate. |
| first_indexed | 2024-09-23T16:24:15Z |
| format | Article |
| id | mit-1721.1/61367 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T16:24:15Z |
| publishDate | 2011 |
| publisher | National Academy of Sciences (U.S.) |
| record_format | dspace |
| spelling | mit-1721.1/613672022-10-02T07:54:51Z A DinB variant reveals diverse physiological consequences of incomplete TLS extension by a Y-family DNA polymerase Walker, Graham C. Jarosz, Daniel F. Delaney, James C. Essigmann, John M. Cohen, Susan E., Ph. D. Massachusetts Institute of Technology Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Chemistry Walker, Graham C. Walker, Graham C. Jarosz, Daniel F. Cohen, Susan E. Delaney, James C. Essigmann, John M. The only Y-family DNA polymerase conserved among all domains of life, DinB and its mammalian ortholog pol κ, catalyzes proficient bypass of damaged DNA in translesion synthesis (TLS). Y-family DNA polymerases, including DinB, have been implicated in diverse biological phenomena ranging from adaptive mutagenesis in bacteria to several human cancers. Complete TLS requires dNTP insertion opposite a replication blocking lesion and subsequent extension with several dNTP additions. Here we report remarkably proficient TLS extension by DinB from Escherichia coli. We also describe a TLS DNA polymerase variant generated by mutation of an evolutionarily conserved tyrosine (Y79). This mutant DinB protein is capable of catalyzing dNTP insertion opposite a replication-blocking lesion, but cannot complete TLS, stalling three nucleotides after an N2[superscript2]-dG adduct. Strikingly, expression of this variant transforms a bacteriostatic DNA damaging agent into a bactericidal drug, resulting in profound toxicity even in a dinB+ background. We find that this phenomenon is not exclusively due to a futile cycle of abortive TLS followed by exonucleolytic reversal. Rather, gene products with roles in cell death and metal homeostasis modulate the toxicity of DinB(Y79L) expression. Together, these results indicate that DinB is specialized to perform remarkably proficient insertion and extension on damaged DNA, and also expose unexpected connections between TLS and cell fate. National Institutes of Health (U.S.) (Grant CA021615) (Grant CA80024) National Institute of Environmental Health Sciences (Grant P30 ES002109) 2011-03-01T23:48:05Z 2011-03-01T23:48:05Z 2009-11 2009-06 Article http://purl.org/eprint/type/JournalArticle 0027-8424 1091-6490 http://hdl.handle.net/1721.1/61367 Jarosz, D. F. et al. “A DinB variant reveals diverse physiological consequences of incomplete TLS extension by a Y-family DNA polymerase.” Proceedings of the National Academy of Sciences 106.50 (2009): 21137-21142. Copyright ©2011 by the National Academy of Sciences https://orcid.org/0000-0001-6159-0778 https://orcid.org/0000-0001-7243-8261 https://orcid.org/0000-0002-2196-5691 en_US http://dx.doi.org/10.1073/pnas.0907257106 Proceedings of the National Academy of Sciences of the United States of America. (PNAS) Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf National Academy of Sciences (U.S.) PNAS |
| spellingShingle | Walker, Graham C. Jarosz, Daniel F. Delaney, James C. Essigmann, John M. Cohen, Susan E., Ph. D. Massachusetts Institute of Technology A DinB variant reveals diverse physiological consequences of incomplete TLS extension by a Y-family DNA polymerase |
| title | A DinB variant reveals diverse physiological consequences of incomplete TLS extension by a Y-family DNA polymerase |
| title_full | A DinB variant reveals diverse physiological consequences of incomplete TLS extension by a Y-family DNA polymerase |
| title_fullStr | A DinB variant reveals diverse physiological consequences of incomplete TLS extension by a Y-family DNA polymerase |
| title_full_unstemmed | A DinB variant reveals diverse physiological consequences of incomplete TLS extension by a Y-family DNA polymerase |
| title_short | A DinB variant reveals diverse physiological consequences of incomplete TLS extension by a Y-family DNA polymerase |
| title_sort | dinb variant reveals diverse physiological consequences of incomplete tls extension by a y family dna polymerase |
| url | http://hdl.handle.net/1721.1/61367 https://orcid.org/0000-0001-6159-0778 https://orcid.org/0000-0001-7243-8261 https://orcid.org/0000-0002-2196-5691 |
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