Novel reference genes for quantifying transcriptional responses of Escherichia coli to protein overexpression by quantitative PCR

Novel reference genes for quantifying transcriptional responses of Escherichia coli to protein overexpression by quantitative PCR

Background: Accurate interpretation of quantitative PCR (qPCR) data requires normalization using constitutively expressed reference genes. Ribosomal RNA is often used as a reference gene for transcriptional studies in E. coli. However, the choice of reliable reference genes has not been systematical...

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Bibliographic Details
Main Authors: Zhou, Kang, Zhou, Lihan, Lim, Qing 'En, Zou, Ruiyang, Stephanopoulos, Gregory, Too, Heng-Phon
Other Authors: Massachusetts Institute of Technology. Department of Chemical Engineering
Format: Article
Language:English
Published: BioMed Central Ltd 2011
Online Access:http://hdl.handle.net/1721.1/64665
https://orcid.org/0000-0001-6909-4568

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http://hdl.handle.net/1721.1/64665
https://orcid.org/0000-0001-6909-4568

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