Potent inhibition of cartilage biosynthesis by coincubation with joint capsule through an IL-1-independent pathway
The reason for the increased risk for development of osteoarthritis (OA) after acute joint trauma is not well understood, but the mechanically injured cartilage may be more susceptible to degradative mediators secreted by other tissues in the joint. To establish a model for such interactions, we coi...
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| Format: | Article |
| Language: | en_US |
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John Wiley & Sons, Inc.
2011
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| Online Access: | http://hdl.handle.net/1721.1/66583 https://orcid.org/0000-0002-4942-3456 |
| _version_ | 1826190105422856192 |
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| author | Grodzinsky, Alan J. Patwari, P. Lin, S. N. Kurz, Bodo Cole, Ada A. Kumar, S. |
| author2 | Massachusetts Institute of Technology. Center for Biomedical Engineering |
| author_facet | Massachusetts Institute of Technology. Center for Biomedical Engineering Grodzinsky, Alan J. Patwari, P. Lin, S. N. Kurz, Bodo Cole, Ada A. Kumar, S. |
| author_sort | Grodzinsky, Alan J. |
| collection | MIT |
| description | The reason for the increased risk for development of osteoarthritis (OA) after acute joint trauma is not well understood, but the mechanically injured cartilage may be more susceptible to degradative mediators secreted by other tissues in the joint. To establish a model for such interactions, we coincubated bovine cartilage tissue explants together with normal joint capsule and found a profound (∼70%) reduction in cartilage proteoglycan biosynthesis. This reduction is due to release by the joint capsule of a heat-labile and non-toxic factor. Surprisingly, while cultured synovium is a canonical source of interleukin-1 (IL-1), blockade either by soluble IL-1 type II receptor (sIL-1r) or IL-1 receptor antagonist (IL-1RA) had no effect. Combined blockade of IL-1 and tumor necrosis factor α (TNF-α) also had no effect. To support the clinical relevance of the findings, we harvested joint capsule from post-mortem human knees. Human joint capsule from a normal adult knee also released a substance that caused an ∼40% decrease in cartilage proteoglycan biosynthesis. Furthermore, this inhibition was not affected by IL-1 blockade with either sIL-1r or IL-1RA. These results suggest that joint capsule tissue from a normal knee joint can release an uncharacterized cytokine that potently inhibits cartilage biosynthetic activity by an IL-1- and TNF-independent pathway. |
| first_indexed | 2024-09-23T08:35:07Z |
| format | Article |
| id | mit-1721.1/66583 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T08:35:07Z |
| publishDate | 2011 |
| publisher | John Wiley & Sons, Inc. |
| record_format | dspace |
| spelling | mit-1721.1/665832022-09-30T09:46:13Z Potent inhibition of cartilage biosynthesis by coincubation with joint capsule through an IL-1-independent pathway Grodzinsky, Alan J. Patwari, P. Lin, S. N. Kurz, Bodo Cole, Ada A. Kumar, S. Massachusetts Institute of Technology. Center for Biomedical Engineering Massachusetts Institute of Technology. Department of Biological Engineering Grodzinsky, Alan J. Grodzinsky, Alan J. Patwari, P. Lin, S. N. The reason for the increased risk for development of osteoarthritis (OA) after acute joint trauma is not well understood, but the mechanically injured cartilage may be more susceptible to degradative mediators secreted by other tissues in the joint. To establish a model for such interactions, we coincubated bovine cartilage tissue explants together with normal joint capsule and found a profound (∼70%) reduction in cartilage proteoglycan biosynthesis. This reduction is due to release by the joint capsule of a heat-labile and non-toxic factor. Surprisingly, while cultured synovium is a canonical source of interleukin-1 (IL-1), blockade either by soluble IL-1 type II receptor (sIL-1r) or IL-1 receptor antagonist (IL-1RA) had no effect. Combined blockade of IL-1 and tumor necrosis factor α (TNF-α) also had no effect. To support the clinical relevance of the findings, we harvested joint capsule from post-mortem human knees. Human joint capsule from a normal adult knee also released a substance that caused an ∼40% decrease in cartilage proteoglycan biosynthesis. Furthermore, this inhibition was not affected by IL-1 blockade with either sIL-1r or IL-1RA. These results suggest that joint capsule tissue from a normal knee joint can release an uncharacterized cytokine that potently inhibits cartilage biosynthetic activity by an IL-1- and TNF-independent pathway. Whitaker Foundation GlaxoSmithKline National Institutes of Health (U.S.) (grant AR-45779) National Institutes of Health (U.S.). Specialized Centers Of Interdisciplinary Research (grant ARP50-39239) 2011-10-26T17:00:39Z 2011-10-26T17:00:39Z 2009-08 Article http://purl.org/eprint/type/JournalArticle 1600-0838 http://hdl.handle.net/1721.1/66583 Patwari, P. et al. “Potent inhibition of cartilage biosynthesis by coincubation with joint capsule through an IL-1-independent pathway.” Scandinavian Journal of Medicine & Science in Sports 19 (2009): 528-535. Web. 26 Oct. 2011. © John Wiley & Sons, Inc. https://orcid.org/0000-0002-4942-3456 en_US http://dx.doi.org/10.1111/j.1600-0838.2009.00911.x Scandinavian Journal of Medicine & Science in Sports Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf John Wiley & Sons, Inc. PubMed Central |
| spellingShingle | Grodzinsky, Alan J. Patwari, P. Lin, S. N. Kurz, Bodo Cole, Ada A. Kumar, S. Potent inhibition of cartilage biosynthesis by coincubation with joint capsule through an IL-1-independent pathway |
| title | Potent inhibition of cartilage biosynthesis by coincubation with joint capsule through an IL-1-independent pathway |
| title_full | Potent inhibition of cartilage biosynthesis by coincubation with joint capsule through an IL-1-independent pathway |
| title_fullStr | Potent inhibition of cartilage biosynthesis by coincubation with joint capsule through an IL-1-independent pathway |
| title_full_unstemmed | Potent inhibition of cartilage biosynthesis by coincubation with joint capsule through an IL-1-independent pathway |
| title_short | Potent inhibition of cartilage biosynthesis by coincubation with joint capsule through an IL-1-independent pathway |
| title_sort | potent inhibition of cartilage biosynthesis by coincubation with joint capsule through an il 1 independent pathway |
| url | http://hdl.handle.net/1721.1/66583 https://orcid.org/0000-0002-4942-3456 |
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