A general screening strategy for peptide-based fluorogenic ligands
A systematic and general approach for identifying efficient probes for class I PDZ domains based on environment-sensitive chromophores is presented. A series of peptides derived from the C-terminal sequence of Stargazin was first used with PDZ domains of PSD-95 and Shank3 to identify the optimal pos...
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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American Chemical Society
2012
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| Online Access: | http://hdl.handle.net/1721.1/69853 https://orcid.org/0000-0002-5749-7869 |
| _version_ | 1826200595151716352 |
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| author | Sainlos, Matthieu Iskenderian, Wendy S. Imperiali, Barbara |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Sainlos, Matthieu Iskenderian, Wendy S. Imperiali, Barbara |
| author_sort | Sainlos, Matthieu |
| collection | MIT |
| description | A systematic and general approach for identifying efficient probes for class I PDZ domains based on environment-sensitive chromophores is presented. A series of peptides derived from the C-terminal sequence of Stargazin was first used with PDZ domains of PSD-95 and Shank3 to identify the optimal position and linker length for the 4-DMAP chromophore. The results were applied to well-characterized ligand sequences for each set of domains to generate high affinity probes that retain their native sequence specificity and yield remarkable fluorescence increases upon binding. These probes constitute efficient tools to study the dynamics and regulatory mechanisms of PDZ domain-mediated interactions. |
| first_indexed | 2024-09-23T11:38:53Z |
| format | Article |
| id | mit-1721.1/69853 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:38:53Z |
| publishDate | 2012 |
| publisher | American Chemical Society |
| record_format | dspace |
| spelling | mit-1721.1/698532022-10-01T04:59:36Z A general screening strategy for peptide-based fluorogenic ligands Sainlos, Matthieu Iskenderian, Wendy S. Imperiali, Barbara Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Chemistry Imperiali, Barbara Imperiali, Barbara Sainlos, Matthieu Iskenderian, Wendy S. A systematic and general approach for identifying efficient probes for class I PDZ domains based on environment-sensitive chromophores is presented. A series of peptides derived from the C-terminal sequence of Stargazin was first used with PDZ domains of PSD-95 and Shank3 to identify the optimal position and linker length for the 4-DMAP chromophore. The results were applied to well-characterized ligand sequences for each set of domains to generate high affinity probes that retain their native sequence specificity and yield remarkable fluorescence increases upon binding. These probes constitute efficient tools to study the dynamics and regulatory mechanisms of PDZ domain-mediated interactions. Human Frontier Science Program (Strasbourg, France) ((RGP0007/2006) National Institutes of Health (U.S.) (NIH Cell Migration Consortium (GM064346)) Marie Curie (Postdoctoral Fellowship (PICK-CPP)) 2012-03-26T14:54:31Z 2012-03-26T14:54:31Z 2009-04 2009-01 Article http://purl.org/eprint/type/JournalArticle 0002-7863 1520-5126 http://hdl.handle.net/1721.1/69853 Sainlos, Matthieu, Wendy S. Iskenderian, and Barbara Imperiali. “A General Screening Strategy for Peptide-Based Fluorogenic Ligands: Probes for Dynamic Studies of PDZ Domain-Mediated Interactions.” Journal of the American Chemical Society 131.19 (2009): 6680–6682. https://orcid.org/0000-0002-5749-7869 en_US http://dx.doi.org/10.1021/ja900371q Journal of the American Chemical Society Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Chemical Society Prof. Imperiali via Erja Kajosalo |
| spellingShingle | Sainlos, Matthieu Iskenderian, Wendy S. Imperiali, Barbara A general screening strategy for peptide-based fluorogenic ligands |
| title | A general screening strategy for peptide-based fluorogenic ligands |
| title_full | A general screening strategy for peptide-based fluorogenic ligands |
| title_fullStr | A general screening strategy for peptide-based fluorogenic ligands |
| title_full_unstemmed | A general screening strategy for peptide-based fluorogenic ligands |
| title_short | A general screening strategy for peptide-based fluorogenic ligands |
| title_sort | general screening strategy for peptide based fluorogenic ligands |
| url | http://hdl.handle.net/1721.1/69853 https://orcid.org/0000-0002-5749-7869 |
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