Identification of host cell factors required for intoxication through use of modified cholera toxin

We describe a novel labeling strategy to site-specifically attach fluorophores, biotin, and proteins to the C terminus of the A1 subunit (CTA1) of cholera toxin (CTx) in an otherwise correctly assembled and active CTx complex. Using a biotinylated N-linked glycosylation reporter peptide attached to...

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Bibliographic Details
Main Authors: Guimaraes, Carla P., Carette, Jan E., Varadarajan, Malini, Antos, John, Spooner, Eric, Brummelkamp, Thijn R., Ploegh, Hidde, Popp, Maximilian W.L.
Other Authors: Massachusetts Institute of Technology. Department of Biology
Format: Article
Language:en_US
Published: Rockefeller University Press 2012
Online Access:http://hdl.handle.net/1721.1/70120
https://orcid.org/0000-0002-1090-6071