Identification of host cell factors required for intoxication through use of modified cholera toxin
We describe a novel labeling strategy to site-specifically attach fluorophores, biotin, and proteins to the C terminus of the A1 subunit (CTA1) of cholera toxin (CTx) in an otherwise correctly assembled and active CTx complex. Using a biotinylated N-linked glycosylation reporter peptide attached to...
Main Authors: | Guimaraes, Carla P., Carette, Jan E., Varadarajan, Malini, Antos, John, Spooner, Eric, Brummelkamp, Thijn R., Ploegh, Hidde, Popp, Maximilian W.L. |
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Other Authors: | Massachusetts Institute of Technology. Department of Biology |
Format: | Article |
Language: | en_US |
Published: |
Rockefeller University Press
2012
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Online Access: | http://hdl.handle.net/1721.1/70120 https://orcid.org/0000-0002-1090-6071 |
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