Enhanced multiplex genome engineering through co-operative oligonucleotide co-selection
Genome-scale engineering of living organisms requires precise and economical methods to efficiently modify many loci within chromosomes. One such example is the directed integration of chemically synthesized single-stranded deoxyribonucleic acid (oligonucleotides) into the chromosome of Escherichia...
| المؤلفون الرئيسيون: | , , , , , , , |
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| مؤلفون آخرون: | |
| التنسيق: | مقال |
| اللغة: | en_US |
| منشور في: |
Oxford University Press (OUP)
2012
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| الوصول للمادة أونلاين: | http://hdl.handle.net/1721.1/71773 https://orcid.org/0000-0002-1332-3197 https://orcid.org/0000-0003-3222-0772 |
| _version_ | 1826206416468180992 |
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| author | Carr, Peter A., Sr. Wang, Harris H. Sterling, Bram Henry Isaacs, Farren J. Lajoie, Marc J. Xu, George Jing Church, George M. Jacobson, Joseph |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Carr, Peter A., Sr. Wang, Harris H. Sterling, Bram Henry Isaacs, Farren J. Lajoie, Marc J. Xu, George Jing Church, George M. Jacobson, Joseph |
| author_sort | Carr, Peter A., Sr. |
| collection | MIT |
| description | Genome-scale engineering of living organisms requires precise and economical methods to efficiently modify many loci within chromosomes. One such example is the directed integration of chemically synthesized single-stranded deoxyribonucleic acid (oligonucleotides) into the chromosome of Escherichia coli during replication. Herein, we present a general co-selection strategy in multiplex genome engineering that yields highly modified cells. We demonstrate that disparate sites throughout the genome can be easily modified simultaneously by leveraging selectable markers within 500 kb of the target sites. We apply this technique to the modification of 80 sites in the E. coli genome. |
| first_indexed | 2024-09-23T13:29:07Z |
| format | Article |
| id | mit-1721.1/71773 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:29:07Z |
| publishDate | 2012 |
| publisher | Oxford University Press (OUP) |
| record_format | dspace |
| spelling | mit-1721.1/717732022-10-01T15:32:04Z Enhanced multiplex genome engineering through co-operative oligonucleotide co-selection Carr, Peter A., Sr. Wang, Harris H. Sterling, Bram Henry Isaacs, Farren J. Lajoie, Marc J. Xu, George Jing Church, George M. Jacobson, Joseph Harvard University--MIT Division of Health Sciences and Technology Massachusetts Institute of Technology. Center for Bits and Atoms Program in Media Arts and Sciences (Massachusetts Institute of Technology) Jacobson, Joseph Carr, Peter A., Sr. Sterling, Bram Henry Xu, George Jing Jacobson, Joseph Genome-scale engineering of living organisms requires precise and economical methods to efficiently modify many loci within chromosomes. One such example is the directed integration of chemically synthesized single-stranded deoxyribonucleic acid (oligonucleotides) into the chromosome of Escherichia coli during replication. Herein, we present a general co-selection strategy in multiplex genome engineering that yields highly modified cells. We demonstrate that disparate sites throughout the genome can be easily modified simultaneously by leveraging selectable markers within 500 kb of the target sites. We apply this technique to the modification of 80 sites in the E. coli genome. United States. Dept. of Energy. Genomes To Life (DE-FG02-03ER6344) National Science Foundation (U.S.). Genes and Genomes Systems Cluster (0719344) National Science Foundation (U.S.). Center for Bits and Atoms (0122419) National Science Foundation (U.S.). Synthetic Biology Engineering Research Center (0540879) 2012-07-24T13:08:14Z 2012-07-24T13:08:14Z 2012-05 2012-03 Article http://purl.org/eprint/type/JournalArticle 0305-1048 1362-4962 http://hdl.handle.net/1721.1/71773 Carr, P. A. et al. “Enhanced Multiplex Genome Engineering Through Co-operative Oligonucleotide Co-selection.” Nucleic Acids Research (2012). https://orcid.org/0000-0002-1332-3197 https://orcid.org/0000-0003-3222-0772 en_US http://dx.doi.org/10.1093/nar/gks455 Nucleic Acids Research Creative Commons Attribution Non-Commercial http://creativecommons.org/licenses/ by-nc/3.0 application/pdf Oxford University Press (OUP) Oxford |
| spellingShingle | Carr, Peter A., Sr. Wang, Harris H. Sterling, Bram Henry Isaacs, Farren J. Lajoie, Marc J. Xu, George Jing Church, George M. Jacobson, Joseph Enhanced multiplex genome engineering through co-operative oligonucleotide co-selection |
| title | Enhanced multiplex genome engineering through co-operative oligonucleotide co-selection |
| title_full | Enhanced multiplex genome engineering through co-operative oligonucleotide co-selection |
| title_fullStr | Enhanced multiplex genome engineering through co-operative oligonucleotide co-selection |
| title_full_unstemmed | Enhanced multiplex genome engineering through co-operative oligonucleotide co-selection |
| title_short | Enhanced multiplex genome engineering through co-operative oligonucleotide co-selection |
| title_sort | enhanced multiplex genome engineering through co operative oligonucleotide co selection |
| url | http://hdl.handle.net/1721.1/71773 https://orcid.org/0000-0002-1332-3197 https://orcid.org/0000-0003-3222-0772 |
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