Methodological considerations for global analysis of cellular FLIM/FRET measurements

Methodological considerations for global analysis of cellular FLIM/FRET measurements

Global algorithms can improve the analysis of fluorescence energy transfer (FRET) measurement based on fluorescence lifetime microscopy. However, global analysis of FRET data is also susceptible to experimental artifacts. This work examines several common artifacts and suggests remedial experimental...

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Bibliographic Details
Main Authors: Rahim, Nur Aida Adbul, Pelet, Serge, Kamm, Roger Dale, So, Peter T. C.
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering
Format: Article
Language:en_US
Published: SPIE 2012
Online Access:http://hdl.handle.net/1721.1/72114
https://orcid.org/0000-0002-7232-304X
https://orcid.org/0000-0003-4698-6488

Internet

http://hdl.handle.net/1721.1/72114
https://orcid.org/0000-0002-7232-304X
https://orcid.org/0000-0003-4698-6488

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