Methodological considerations for global analysis of cellular FLIM/FRET measurements
Global algorithms can improve the analysis of fluorescence energy transfer (FRET) measurement based on fluorescence lifetime microscopy. However, global analysis of FRET data is also susceptible to experimental artifacts. This work examines several common artifacts and suggests remedial experimental...
| Main Authors: | , , , |
|---|---|
| 其他作者: | |
| 格式: | 文件 |
| 语言: | en_US |
| 出版: |
SPIE
2012
|
| 在线阅读: | http://hdl.handle.net/1721.1/72114 https://orcid.org/0000-0002-7232-304X https://orcid.org/0000-0003-4698-6488 |