Functional genomics, proteomics, and regulatory DNA analysis in isogenic settings using zinc finger nuclease-driven transgenesis into a safe harbor locus in the human genome
Isogenic settings are routine in model organisms, yet remain elusive for genetic experiments on human cells. We describe the use of designed zinc finger nucleases (ZFNs) for efficient transgenesis without drug selection into the PPP1R12C gene, a “safe harbor” locus known as AAVS1. ZFNs enable target...
Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Other Authors: | |
Format: | Article |
Language: | en_US |
Published: |
Cold Spring Harbor Laboratory Press
2012
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Online Access: | http://hdl.handle.net/1721.1/72607 https://orcid.org/0000-0002-3829-5612 https://orcid.org/0000-0002-1446-7256 |