Enhancing Protease Activity Assay in Droplet-Based Microfluidics Using a Biomolecule Concentrator
We introduce an integrated microfluidic device consisting of a biomolecule concentrator and a microdroplet generator, which enhances the limited sensitivity of low-abundance enzyme assays by concentrating biomolecules before encapsulating them into droplet microreactors. We used this platform to det...
| Main Authors: | , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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American Chemical Society (ACS)
2012
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| Online Access: | http://hdl.handle.net/1721.1/73163 https://orcid.org/0000-0002-1801-5548 https://orcid.org/0000-0001-7215-1439 |
| _version_ | 1826198332984262656 |
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| author | Chen, Chia-Hung Sarkar, Aniruddh Song, Yong-Ak Miller, Miles Aaron Kim, Sung Jae Griffith, Linda G. Lauffenburger, Douglas A. Han, Jongyoon |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Chen, Chia-Hung Sarkar, Aniruddh Song, Yong-Ak Miller, Miles Aaron Kim, Sung Jae Griffith, Linda G. Lauffenburger, Douglas A. Han, Jongyoon |
| author_sort | Chen, Chia-Hung |
| collection | MIT |
| description | We introduce an integrated microfluidic device consisting of a biomolecule concentrator and a microdroplet generator, which enhances the limited sensitivity of low-abundance enzyme assays by concentrating biomolecules before encapsulating them into droplet microreactors. We used this platform to detect ultralow levels of matrix metalloproteinases (MMPs) from diluted cellular supernatant and showed that it significantly (~10-fold) reduced the time required to complete the assay and the sample volume used. |
| first_indexed | 2024-09-23T11:03:12Z |
| format | Article |
| id | mit-1721.1/73163 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:03:12Z |
| publishDate | 2012 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/731632022-09-27T16:46:33Z Enhancing Protease Activity Assay in Droplet-Based Microfluidics Using a Biomolecule Concentrator Chen, Chia-Hung Sarkar, Aniruddh Song, Yong-Ak Miller, Miles Aaron Kim, Sung Jae Griffith, Linda G. Lauffenburger, Douglas A. Han, Jongyoon Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Goyal, Chia-Hung Sarkar, Aniruddh Song, Yong-Ak Miller, Miles Aaron Kim, Sung Jae Griffith, Linda G. Lauffenburger, Douglas A. Han, Jongyoon We introduce an integrated microfluidic device consisting of a biomolecule concentrator and a microdroplet generator, which enhances the limited sensitivity of low-abundance enzyme assays by concentrating biomolecules before encapsulating them into droplet microreactors. We used this platform to detect ultralow levels of matrix metalloproteinases (MMPs) from diluted cellular supernatant and showed that it significantly (~10-fold) reduced the time required to complete the assay and the sample volume used. National Institutes of Health (U.S.) (Grant GM68762) National Institutes of Health (U.S.) (Grant U54-CA112967) National Institutes of Health (U.S.) (Grant R01-EB010246) National Institutes of Health (U.S.) (Grant R01-GM081336) National Science Foundation (U.S.) (Graduate Fellowship) United States. Defense Advanced Research Projects Agency (Cipher Program) 2012-09-25T14:05:25Z 2012-09-25T14:05:25Z 2011-06 2011-04 Article http://purl.org/eprint/type/JournalArticle 0002-7863 1520-5126 http://hdl.handle.net/1721.1/73163 Chen, Chia-Hung et al. “Enhancing Protease Activity Assay in Droplet-Based Microfluidics Using a Biomolecule Concentrator.” Journal of the American Chemical Society 133.27 (2011): 10368–10371. https://orcid.org/0000-0002-1801-5548 https://orcid.org/0000-0001-7215-1439 en_US http://dx.doi.org/10.1021/ja2036628 Journal of the American Chemical Society Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf American Chemical Society (ACS) PubMed Central |
| spellingShingle | Chen, Chia-Hung Sarkar, Aniruddh Song, Yong-Ak Miller, Miles Aaron Kim, Sung Jae Griffith, Linda G. Lauffenburger, Douglas A. Han, Jongyoon Enhancing Protease Activity Assay in Droplet-Based Microfluidics Using a Biomolecule Concentrator |
| title | Enhancing Protease Activity Assay in Droplet-Based Microfluidics Using a Biomolecule Concentrator |
| title_full | Enhancing Protease Activity Assay in Droplet-Based Microfluidics Using a Biomolecule Concentrator |
| title_fullStr | Enhancing Protease Activity Assay in Droplet-Based Microfluidics Using a Biomolecule Concentrator |
| title_full_unstemmed | Enhancing Protease Activity Assay in Droplet-Based Microfluidics Using a Biomolecule Concentrator |
| title_short | Enhancing Protease Activity Assay in Droplet-Based Microfluidics Using a Biomolecule Concentrator |
| title_sort | enhancing protease activity assay in droplet based microfluidics using a biomolecule concentrator |
| url | http://hdl.handle.net/1721.1/73163 https://orcid.org/0000-0002-1801-5548 https://orcid.org/0000-0001-7215-1439 |
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