Surface-engineered substrates for improved human pluripotent stem cell culture under fully defined conditions

The current gold standard for the culture of human pluripotent stem cells requires the use of a feeder layer of cells. Here, we develop a spatially defined culture system based on UV/ozone radiation modification of typical cell culture plastics to define a favorable surface environment for human plu...

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Bibliographic Details
Main Authors: Saha, Krishanu, Mei, Ying, Reisterer, Colin M., Pyzocha, Neena Kenton, Yang, Jing, Muffat, Julien, Davies, Martyn C., Alexander, Morgan R., Jaenisch, Rudolf, Langer, Robert S, Anderson, Daniel Griffith
Other Authors: Harvard University--MIT Division of Health Sciences and Technology
Format: Article
Language:en_US
Published: National Academy of Sciences 2012
Online Access:http://hdl.handle.net/1721.1/74094
https://orcid.org/0000-0001-5629-4798
https://orcid.org/0000-0003-4255-0492