Genome-wide identification of Ago2 binding sites from mouse embryonic stem cells with and without mature microRNAs

Genome-wide identification of Ago2 binding sites from mouse embryonic stem cells with and without mature microRNAs

MicroRNAs (miRNAs) are 19–22-nucleotide noncoding RNAs that post-transcriptionally regulate mRNA targets. We have identified endogenous miRNA binding sites in mouse embryonic stem cells (mESCs), by performing photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by de...

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Bibliographic Details
Main Authors: Leung, Anthony, Young, Amanda G., Bhutkar, Arjun (AJ), Zheng, Grace X., Bosson, Andrew David, Nielsen, Cydney B., Sharp, Phillip A.
Other Authors: Massachusetts Institute of Technology. Computational and Systems Biology Program
Format: Article
Language:en_US
Published: Nature Publishing Group 2012
Online Access:http://hdl.handle.net/1721.1/74622
https://orcid.org/0000-0003-1465-1691

Internet

http://hdl.handle.net/1721.1/74622
https://orcid.org/0000-0003-1465-1691

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