Retargeting of pre-set regions on chromosome for high gene expression in mammalian cells
We have developed a system to hunt and reuse special gene integration sites that allow for high and stable gene expression. A vector, named pRGFP8, was constructed. The plasmid pRGFP8 contains a reporter gene, gfp2 and two extraneous DNA fragments. The gene gfp2 makes it possible to screen the high...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
2004
|
Subjects: | |
Online Access: | http://hdl.handle.net/1721.1/7491 |