Retargeting of pre-set regions on chromosome for high gene expression in mammalian cells

We have developed a system to hunt and reuse special gene integration sites that allow for high and stable gene expression. A vector, named pRGFP8, was constructed. The plasmid pRGFP8 contains a reporter gene, gfp2 and two extraneous DNA fragments. The gene gfp2 makes it possible to screen the high...

Full description

Bibliographic Details
Main Authors: Jiao, Peng, Chang, Christine, Kral, Kelly, Rogg, Jonathan, Wyhs, Nicolas, Wang, Daniel I.C.
Format: Article
Language:English
Published: 2004
Subjects:
Online Access:http://hdl.handle.net/1721.1/7491