Microfluidic Preparative Free-Flow Isoelectric Focusing: System Optimization for Protein Complex Separation
Isoelectric focusing (IEF) is the first step for two-dimensional (2D) gel electrophoresis and plays an important role in sample purification for proteomics. However, biases in protein size and pI resolution, as well as limitations in sample volume, gel capacity, sample loss, and experimental time, r...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
American Chemical Society (ACS)
2012
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| Online Access: | http://hdl.handle.net/1721.1/75011 https://orcid.org/0000-0002-9547-3251 https://orcid.org/0000-0001-7192-580X |