Single molecule fluorescent in situ hybridization (smFISH) of C. elegans worms and embryos

In C. elegans, the expression pattern of a gene provides important clues to understanding its biological function. To accurately depict endogenous transcriptional activity, a highly sensitive method is required to measure transcript levels in the intact tissue across various developmental stages. Co...

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Bibliographic Details
Main Authors: Ji, Ni, van Oudenaarden, Alexander
Other Authors: Massachusetts Institute of Technology. Department of Biology
Format: Book chapter
Language:en_US
Published: WormBook 2013
Online Access:http://hdl.handle.net/1721.1/76660