Preparation of unnatural N-to-N and C-to-C protein fusions

Standard genetic approaches allow the production of protein composites by fusion of polypeptides in head-to-tail fashion. Some applications would benefit from constructions that are genetically impossible, such as the site-specific linkage of proteins via their N or C termini, when a remaining free...

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Bibliographic Details
Main Authors: Witte, Martin D., Cragnolini, Juan J., Dougan, Stephanie K., Yoder, Nicholas C., Ploegh, Hidde, Popp, Maximilian W.L.
Other Authors: Massachusetts Institute of Technology. Department of Biology
Format: Article
Language:en_US
Published: National Academy of Sciences (U.S.) 2013
Online Access:http://hdl.handle.net/1721.1/76755
https://orcid.org/0000-0002-1090-6071