In vitro and in vivo mRNA delivery using lipid-enveloped pHresponsive polymer nanoparticles
Biodegradable core−shell structured nanoparticles with a poly(β-amino ester) (PBAE) core enveloped by a phospholipid bilayer shell were developed for in vivo mRNA delivery with a view toward delivery of mRNA-based vaccines. The pH-responsive PBAE component was chosen to promote endosome disruption,...
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| Format: | Article |
| Language: | en_US |
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American Chemical Society
2013
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| Online Access: | http://hdl.handle.net/1721.1/79764 |
| _version_ | 1811090974175133696 |
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| author | Su, Xingfang Fricke, Jennifer Kavanagh, Daniel G. Irvine, Darrell J |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Su, Xingfang Fricke, Jennifer Kavanagh, Daniel G. Irvine, Darrell J |
| author_sort | Su, Xingfang |
| collection | MIT |
| description | Biodegradable core−shell structured nanoparticles with a poly(β-amino ester) (PBAE) core enveloped by a phospholipid bilayer shell were developed for in vivo mRNA delivery with a view toward delivery of mRNA-based vaccines. The pH-responsive PBAE component was chosen to promote endosome disruption, while the lipid surface layer was selected to minimize toxicity of the polycation core. Messenger RNA was efficiently adsorbed via electrostatic interactions onto the surface of these net positively charged nanoparticles. In vitro, mRNA-loaded particle uptake by dendritic cells led to mRNA delivery into the cytosol with low cytotoxicity, followed by translation of the encoded protein in these difficult-to-transfect cells at a frequency of 30%. Particles loaded with mRNA administered intranasally (i.n.) in mice led to the expression of the reporter protein luciferase in vivo as soon as 6 h after administration, a time point when naked mRNA given i.n. showed no expression. At later time points, luciferase expression was detected in naked mRNA-treated mice, but this group showed a wide variation in levels of transfection, compared to particle-treated mice. This system may thus be promising for noninvasive delivery of mRNA-based vaccines. |
| first_indexed | 2024-09-23T14:55:07Z |
| format | Article |
| id | mit-1721.1/79764 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:55:07Z |
| publishDate | 2013 |
| publisher | American Chemical Society |
| record_format | dspace |
| spelling | mit-1721.1/797642022-09-29T11:23:50Z In vitro and in vivo mRNA delivery using lipid-enveloped pHresponsive polymer nanoparticles Su, Xingfang Fricke, Jennifer Kavanagh, Daniel G. Irvine, Darrell J Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Materials Science and Engineering Ragon Institute of MGH, MIT and Harvard Koch Institute for Integrative Cancer Research at MIT Su, Xingfang Fricke, Jennifer Kavanagh, Daniel G. Irvine, Darrell J. Biodegradable core−shell structured nanoparticles with a poly(β-amino ester) (PBAE) core enveloped by a phospholipid bilayer shell were developed for in vivo mRNA delivery with a view toward delivery of mRNA-based vaccines. The pH-responsive PBAE component was chosen to promote endosome disruption, while the lipid surface layer was selected to minimize toxicity of the polycation core. Messenger RNA was efficiently adsorbed via electrostatic interactions onto the surface of these net positively charged nanoparticles. In vitro, mRNA-loaded particle uptake by dendritic cells led to mRNA delivery into the cytosol with low cytotoxicity, followed by translation of the encoded protein in these difficult-to-transfect cells at a frequency of 30%. Particles loaded with mRNA administered intranasally (i.n.) in mice led to the expression of the reporter protein luciferase in vivo as soon as 6 h after administration, a time point when naked mRNA given i.n. showed no expression. At later time points, luciferase expression was detected in naked mRNA-treated mice, but this group showed a wide variation in levels of transfection, compared to particle-treated mice. This system may thus be promising for noninvasive delivery of mRNA-based vaccines. United States. Dept. of Defense (Institute for Soldier Nanotechnology, contract W911NF-07-D-0004) Ragon Institute of MGH, MIT and Harvard Singapore. Agency for Science, Technology and Research Howard Hughes Medical Institute (Investigator) 2013-08-02T16:27:50Z 2013-08-02T16:27:50Z 2011-06 2011-02 Article http://purl.org/eprint/type/JournalArticle 1543-8384 1543-8392 http://hdl.handle.net/1721.1/79764 Su, Xingfang, Jennifer Fricke, Daniel G. Kavanagh, and Darrell J. Irvine. “In Vitro and in Vivo mRNA Delivery Using Lipid-Enveloped pH-Responsive Polymer Nanoparticles.” Molecular Pharmaceutics 8, no. 3 (June 6, 2011): 774-787. en_US http://dx.doi.org/10.1021/mp100390w Molecular Pharmaceutics Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf American Chemical Society PubMed Central |
| spellingShingle | Su, Xingfang Fricke, Jennifer Kavanagh, Daniel G. Irvine, Darrell J In vitro and in vivo mRNA delivery using lipid-enveloped pHresponsive polymer nanoparticles |
| title | In vitro and in vivo mRNA delivery using lipid-enveloped pHresponsive polymer nanoparticles |
| title_full | In vitro and in vivo mRNA delivery using lipid-enveloped pHresponsive polymer nanoparticles |
| title_fullStr | In vitro and in vivo mRNA delivery using lipid-enveloped pHresponsive polymer nanoparticles |
| title_full_unstemmed | In vitro and in vivo mRNA delivery using lipid-enveloped pHresponsive polymer nanoparticles |
| title_short | In vitro and in vivo mRNA delivery using lipid-enveloped pHresponsive polymer nanoparticles |
| title_sort | in vitro and in vivo mrna delivery using lipid enveloped phresponsive polymer nanoparticles |
| url | http://hdl.handle.net/1721.1/79764 |
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