M13 Bacteriophage Display Framework That Allows Sortase-Mediated Modification of Surface-Accessible Phage Proteins

M13 Bacteriophage Display Framework That Allows Sortase-Mediated Modification of Surface-Accessible Phage Proteins

We exploit bacterial sortases to attach a variety of moieties to the capsid proteins of M13 bacteriophage. We show that pIII, pIX, and pVIII can be functionalized with entities ranging from small molecules (e.g., fluorophores, biotin) to correctly folded proteins (e.g., GFP, antibodies, streptavidin...

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Bibliographic Details
Main Authors: Hess, Gaelen T., Cragnolini, Juan J., Popp, Maximilian W., Allen, Mark A., Dougan, Stephanie K., Spooner, Eric, Ploegh, Hidde L., Belcher, Angela M., Guimaraes, Carla P.
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering
Format: Article
Language:en_US
Published: American Chemical Society (ACS) 2013
Online Access:http://hdl.handle.net/1721.1/80400
https://orcid.org/0000-0001-9353-7453
https://orcid.org/0000-0002-1090-6071

Internet

http://hdl.handle.net/1721.1/80400
https://orcid.org/0000-0001-9353-7453
https://orcid.org/0000-0002-1090-6071

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